Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-09-17
1998-10-06
Campbell, Eggerton A.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, C07H 202, C12Q 168
Patent
active
058177957
ABSTRACT:
Compounds referred to herein as oligonucleotide clamps are provided that stably bind to target polynucleotides in a sequence-specific manner. The oligonucleotide clamps comprise one or more oligonucleotide moieties capable of specifically binding to a target polynucleotide and one or more pairs of binding moieties covalently linked to the oligonucleotide moieties. In accordance with the invention, upon annealing of the oligonucleotide moieties to the target polynucleotide, the binding moieties of a pair are brought into juxtaposition so that they form a stable covalent or non-covalent linkage or complex. The interaction of the binding moieties of the one or more pairs effectively clamps the specifically annealed oligonucleotide moieties to the target polynucleotide.
REFERENCES:
patent: 4599303 (1986-07-01), Yabusaki et al.
patent: 4739044 (1988-04-01), Stabinsky et al.
patent: 4948882 (1990-08-01), Ruth
patent: 4958013 (1990-09-01), Letsinger
patent: 5093232 (1992-03-01), Urden et al.
patent: 5098890 (1992-03-01), Gewirtz et al.
patent: 5166315 (1992-11-01), Summerton et al.
patent: 5473060 (1995-12-01), Gryaznov et al.
Agrawal, et al., "Site specific functionalization of oligonucleotides for attaching two different reporter groups," Nuc. Acids Res. 18:5419-5423 (1990).
Agrawal, et al., "Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling," Tetrahedron Lett. 31:1543-1546 (1990).
Ashley, et al., "Chemical synthesis of oligonucleotide dumb-bells, " Biochem 30:2927-2933 (1991).
Beal, et al., "Recognition of double helical DNA by alternate strand triple helix formation," J. Am. Chem. Soc. 114:4976-4982 (1992).
Blume, et al., "Triple helix formation by purine-rich oligonucleotides targeted to the human diydrofolate reductase promoter, " Nuc. Acids Res. 20:1777-1784 (1992).
Clontech Laboratories 1989-1990 Catalog (excerpt).
Durand, et al., "Circular dichroism studies of an oligonucleotide containing a hairpin loop made of a hexaethylene glycolchain: conformation and stability," Nuc. Acids Res. 18:6353-6359 (1990).
Farooqui, et al., "Effect of structural variations in cholesteryl-conjugated oligonucleotides on inhibitory activity towards HIV-1," Bioconjugate Chem. 2:422-426 (1991).
Ferris, et al., "4-cyanomidazole and diimidazole imine: water-soluble condensing agents for the formation of the phosphodiester bond," Nucleosides & Nucleotides 8:407-414 (1989).
Francois, et al., "Recognition and cleavage of duplex DNA via trip-helix formation by oligonucleotides covalently linked to a phenathroline-copper chelate," Proc. Natl. Acad. Sci. 86:9702-9706 (1989).
Froehler, et al., "Phosphoramidate analogues of DNA: synthesis and thermal stability of heteroduplexes," Nuc. Acids Res. 16:4831-4839 (1989).
Goodchild, "Conjugates of oligonucleotides and modified oligonucleptodes: a review of their synthesis and properties," Bioconjugate Chem. 1;165-187 (1990).
Gryaznov, et al., "Chemical ligation of oligonucleotides in the presence and absence of a template," J. Am. Chem. Soc. 115:3808-3809 (1993).
Gryaznov, et al., Synthesis and properties of oligonucleotides containing aminodeoxythymidine units, Nuc. Acids Res. 20:3403-3409 (1992).
Gryaznov, et al., "Template controlled coupling and recombination of oligonucleotide blocks containing thiophosphoryl groups," Nuc. Acids Res. 21:1403-1408 (1993).
Gryaznov, et al., "Anchor for one step release of 3'-amino-oligonucleotides from a solid support," Tetrahedron Lett. 34:1261-1264 (1993).
Jager, et al., "Oligonucleotide 4-alkylphosphoramidates: synthesis and binding to polynucleotides," Biochem. 27:7237-7246 (1988).
Jaschke, et al., "Automated incorporation of polyethylene glycol into synthetic oligonucleotides," Tetrahedron Lett. 34:301-304.
Kabanov, et al., "A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibits influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells," FEBS Lett. 259:327-330 (1990).
Knorre, et al., "Novel antisense derivatives: antisense DNA intercalators, cleavers, and alkylators," in Prospects for Antisense Nucleic Acid Therapy of Cancer and AIDS (Wiley-Liss, New York, NY) pp. 195-218 (1991).
Letsinger, et al., "Synthesis and properties of modified oligonucleotides," Nuc. Acids Res. 24:75-78 (1991).
Letsinger, et al., "Cholesteryl-conjugated oligonucleotides: synthesis, properties, and activity as inhibitors of replication of human immunodeficiency virus in cell culture," Proc. Natl. Acad. Sci. 86:6553-6556 (1989).
Luebke, et al., "Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation," J. Am. Chem. Soc. 111:8733-8735 (1989).
Luebke, et al., "Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation," Nuc. Acids Res. 20:3005-3009 (1992).
Ma, et al., "Design and synthesis of RNA miniduplexes via a synthetic linker approach," Biochem. 32:1751-1758 (1993).
MacKellar, et al., "Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups," Nuc. Acids Res. 20:3411-3417 (1992).
Mitsuya, H., et al., "Long-Term Inhibition of Human T-Lympho-tropic Virus Type III/Lymphadenopathy-Associated Virus (Human Immunodeficiency Virus) DNA Synthesis and RNA Expression in T Cells Protected by 2',3'-Dideoxynucleosides in Vitro" Proc. Natl. Acad. Sci. USA 84:2033-2037 (1987).
Mitsuya, H., et al., "3 '-Azido-3'-Deoxythymidine (BW A509U) : An Antiviral Agent that Inhibits the Infectivity and Cytopathic Effect of Human T-Lymphotropic Virus type III/Lymphadenopathy-Associated Virus in Vitro" Proc. Natl. Acad. Sci. USA 82:7096-7100 (1985).
Mitsuya, H., and Broder, S., "Inhibition of the in Vitro Infectivity and Cytopathic Effect of Human T-Lymphotrophic Virus Type III/Lymphadenopathy-Associated Virus (HTLV-III/LAV) by 2',3'-Dideoxynucleosides" Proc. Natl. Acad. Sci. USA 83:1911-1915 (1986).
Mitsuya, H., and Yarchoan, R., "Development of Antiretroviral Therapy for AIDS and Related Disorders" in Textbook of AIDS Medicine (Broder, S., et al., Eds.) Williams & Wilkins, Baltimore, MD, pp. 721-742, Section V., Chapter 47 (1994).
Newton, et al., "The production of PCR products with 5' single-stranded tails using primers that incorporated novel phosphoramidite intermediates," Nuc. Acids Res. 21:1155-1162 (1993).
Reed, et al., "Acridine-and cholesterol-derivatized solid supports for improved synthesis of 14-modified oligonucleotides," Bioconjugate Chem. 2:217-225 (1991).
Roberts, et al., "Specificity and stringency in DNA triplex formation," Proc. Natl. Acad. Sci. 88:9397-9401 (1991).
Rothenberg, M., et al., "Oligonucleotides as Anti-Sense Inhibitors of Gene Expression: Therapeutic Implications," J. Natl. Cancer Inst. 81:1539-1544 (1989).
Salunkhe, et al., "Control of folding and binding of oligonucleotides by use of a nonnucleotide linker," J. Am. Chem. Soc. 114:8768-8772 (1992).
Shabarova, et al., "Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene," Nuc. Acids Res. 19:4247-4251 (1991).
Shea, et al., "Synthesis, hybridization properties and antiviral activity of lipid-oligonucleotide conjugates," Nuc. Acids Res. 18:3777-3783 (1990).
Simons, et al., "Antisense c-myb Oligonucleotides Inhibit Intimal Arterial Smooth Muscle Cell accumulation in Vivo,"Nature 359:67-70 (1992).
Sokolova, et al., "Chemical reactions within DNA duplexes: cyanogen bromide as an effective oligodeoxynucleotide coupling agent," FEBS Lett. 232:153-155 (1988).
Stein, et al., "Mode of action of 5'-linked cholesteryl phosphorothioate oligonucleotides in inhibiting syncytia formation and infection by HIV-1 and HIV-2 in vitro," Biochem. 30:2439-2444 (1991).
Thuong, et al., "Oligonucleotides attached to intercalators, photoreactive and cleavage agents," in Oligonucleotides and Analogues: A Practical Approach (Eckstein, Ed.; IRL Press, Oxford, U.K.) (1991).
Thoung, et al., "Syntheses et reactivite d'oligothymidylates substitues par un agent intercalant et un groupe thiophosphate,"Tetrahedron Lett. 28:4157-4160 (1987).
Uhlmann,
Gryaznov Sergei M.
Lloyd David H.
Campbell Eggerton A.
Lynx Therapeutics, Inc.
Macevicz Stephen C.
Powers Vincent M.
LandOfFree
Oligonucleotide clamps having diagnostic and therapeutic applica does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Oligonucleotide clamps having diagnostic and therapeutic applica, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide clamps having diagnostic and therapeutic applica will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-78752