Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-13
1998-04-14
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 221, 536 243, 536 2431, C12Q 168, C07H 1900, C07H 2104
Patent
active
057389934
ABSTRACT:
An oligonucleotide comprising a specific region and one or two non-specific regions which are bound to at least one of the two termini of the specific region, wherein the specific region has a specific base sequence which is substantially complementary to a target sequence of a sample nucleic acid with which the oligonucleotide is to be hybridized, the non-specific region is composed of at least one nucleotide having other base capable of forming a base pair with each of bases constituting a common nucleic acid, or its oligomer, is used as a probe for the hybridization.
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patent: 5187085 (1993-02-01), Lee
Brennan et al, (1986), "The effects of base analogue substitutions on the cleavage by the EcoRI restriction endonuclease of octadeoxyribonucleotides containing modified EcoRI recognition sequences", J. Biol. Chem. 261(16):7270-7278.
Ohtsuka et al, (1985), "An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions", J. Biol. Chem. 260(5):2605-2608.
Knoth et al, (1988), "Highly degenerate, inosine containing primer specifically amplify rare cDNA using the polymerase chain reaction", Nucleic Acids Res. 16(22):10932.
Stahl et al, (1978), "Transcription of T7 DNA containing modified nucleotides by bacteriophage T7 specific RNA polymerase", J. Biol. Chem. 253(14):4951-4959.
Martin et al, (1985), "Base pairing involving deoxyinosine:implications for probe design", Nucleic Acids Res. 13(24):8927-8938.
Dianov et al, (1991), "Preferential recognition of I-T base-pairs in the initiation of excision repair by hypoxanthine-DNA glycosylase", Nucleic Acids Res. 19(14):3829-3833.
Lin et al, (1992), "Synthesis of oligodeoxyribonucleotides containing degenerate bases and their use as primers in the polymerase chain reaction", Nucleic Acids Res. 20(19) 5149-5152.
Fugono Nobutake
Kurusu Yasurou
Terasawa Masato
Yukawa Hideaki
Marschel Ardin H.
Mitsubishi Chemical Corporation
Riley Jezia
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