Oligodeoxynucleotide probes for Campylobacter fetus and Campylob

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435 6, 435 29, C07H 1512, C12Q 168, C12Q 102

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052024253

ABSTRACT:
Single-stranded DNA probes complementary to a hypervariable region of Campylobacter 16S rRNA are useful in distinguishing species of this pathogen from one another. The probes find practical application in diagnosing human and animal diseases caused by this organism from clinical samples, such as fecal material. They are also useful in differentiating Campylobacter species based on RFLP analyses.

REFERENCES:
Louis M. Thompson III et al., "Phylogenetic Study of the Genus Campylobacter," Int. J. System. Bacteriol. 38(2): 190-200 (Apr. 1988).
Paul P. Lau et al., "Phylogenetic Diversity and Position of the Genus Campylobacter," System. Appl. Microbiol. 9: 231-238 (1987).
Paul J. Romaniuk et al., "Identification of Campylobacter Species by Southern Hybridization of Genomic DNA Using an Oligonucleotide Probe for 16S rRNA Genes," FEMS Microbiol. Lett. 43: 331-335 (1987).
C. J. Gebhart et al., "Species-Specific Cloned DNA Probes for the Identification of Campylobacter hyointestinalis," J. Clin. Microbiol. 27(12): 2717-2723 (Dec. 1989).
E. J. Schrawder et al., "Detection of Campylobacter by Colony Hybridization With Colorimetric Oligonucleotide Probes," Abstr. 113, Clin. Chem. 34(6): 1176 (1988).
P. Moreau et al., "Campylobacter Species Identification Based on Polymorphism of DNA Encoding rRNA," J. Clin. Microbiol. 27(7): 1514-1517 (Jul. 1989).
I. V. Wesley et al., "Development of Nucleic Acid Probes for Campylobacter fetus and Campylobacter hyointestinalis," Abstract for 1989 Annual meeting of American Assoc. of Vet. Lab. Diagnost., Oct. 28-31, 1989, Las Vegas, NV.
Irene V. Wesley, "Nucleic Acid Probes for Campylobacter fetus and Campylobacter hyointestinalis Based on 16S Ribosomal RNA (16S rRNA) Analysis," Abstr. Cl 302, 19th Annual Meeting, UCLA Symposia on Molecular & Cellular Biology, Feb. 3-Mar. 11, 1990 (J. Cell. Biochem.).

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