Oligodendrocyte precursor cell composition and methods of use

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se

Reexamination Certificate

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C435S325000, C435S374000

Reexamination Certificate

active

07985586

ABSTRACT:
The present invention provides a cell culture enriched for sphingolipid enhances neural stem cells (SENSe), particularly oligodendrocyte precursor cells (ODPCs), that do not form teratomas after transplanted in vivo. Methods for producing and use of the invention ODPCs or the cell culture enriched with these ODPCs for stem cell therapy are also provided. The invention method comprises culturing a stem cell culture with a cell culture medium comprising a ceramide compound and a S1P receptor agonist in sequence, overlapping intervals or concurrent manners. The present invention further provides a cellular or gene therapy using a composition comprising a ceramide compound in conjunction with a S1P1agonist to proliferate or differentiate endogeneous neural stem cells to ODPCs and further to oligodendrocytes.

REFERENCES:
patent: WO00/09139 (2000-02-01), None
patent: WO2004/029203 (2004-04-01), None
patent: WO2007/056505 (2007-05-01), None
Yamada et al, 1996. Development and differentiation of oligodendrocytes. Japanese Journal of Physiology, vol. 46: 105-110.
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Bieberich, 2008, “Smart Drugs for Smarter Stem Cells: Making SENSe (Sphingolipid-Enhanced Neural Stem Cells) of Ceramide,” Neuro-Signals, vol. 16(2-3):124-139.
Clemens et al., “Synthesis of 4(5)-Phenylimidazole-Based Analogues of Sphingosine-1-Phosphate and FTY720: Discovery of Potent S1P1 Receptor Agonists,” Bioorganic & Medicinal Chemistry Letters, vol. 15(15):3568-3572, 2005.
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Santos et al., 2004, “Synthesis and Biological Evaluation of Phosphonic and Thiophosphoric Acid Derivatives of Lysophosphatidic Acid,” Bioorganic & Medicinal Chemistry Letters, vol. 14(13):3473-3476.
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PCT International Search Report for PCT/US20091033056 dated Jul. 10, 2009 (8 pages).

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