Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-01-31
2006-01-31
Brusca, John S. (Department: 1631)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S025320
Reexamination Certificate
active
06992180
ABSTRACT:
The present invention provides a nucleotide having the formula,in-line-formulae description="In-line Formulae" end="lead"?Sig-PM-SM-BASEin-line-formulae description="In-line Formulae" end="tail"?wherein PM is a phosphate moiety, SM is a sugar moiety and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached to the 3′ or the 5′ position of the sugar moiety when the nucleotide is a deoxyribonucleotide and at the 2′, 3′ or 5′ position when the nucleotide is a ribonucleotide. BASE is attached to the 1′ position of SM from the N1position when BASE is a pyrmidine or the N9position when BASE is a purine or 7-deazapurine. Sig is covalently attached to PM directly or via a chemical linkage, and represents a detectable moiety covalently attached to SM directly or through a linkage group. Also provided are an oligo- or polynucleotide comprising at least one such phosphate-moiety labeled nucleotide, and other compositions including those wherein a polypeptide is terminally ligated or attached to the oligo- or polynucleotide. The phosphate-moiety labeled nucleotide, and the oligo- or polynucleotides and other compositions comprising such phosphate-moiety labeled nucleotides, are useful as diagnostic tools for detecting analytes and as therapeutic agents.
REFERENCES:
patent: 4224408 (1980-09-01), Hung et al.
patent: 4260737 (1981-04-01), Scherberg
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4378458 (1983-03-01), Gohlke et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4847240 (1989-07-01), Ryser et al.
patent: 5212059 (1993-05-01), Schwartz et al.
patent: 5242906 (1993-09-01), Pagano et al.
patent: 5591600 (1997-01-01), Ecker, et al
patent: 5591720 (1997-01-01), Anderson et al.
patent: 5614617 (1997-03-01), Cook et al.
patent: 5643730 (1997-07-01), Banker et al.
patent: 5643780 (1997-07-01), Baker
patent: 5652094 (1997-07-01), Usman et al.
patent: 5665710 (1997-09-01), Rahman et al.
patent: 5706498 (1998-01-01), Fujimiya et al.
patent: 5736294 (1998-04-01), Ecker, et al.
patent: 5807677 (1998-09-01), Eigen et al.
patent: 5811232 (1998-09-01), Crooke et al.
patent: 5874564 (1999-02-01), Ecker, et al.
patent: 5891468 (1999-04-01), Martin et al.
patent: 5914230 (1999-06-01), Liu et al.
patent: 5953727 (1999-09-01), Maslyn et al.
patent: 5966712 (1999-10-01), Sabatini et al.
patent: 5980096 (1999-11-01), Thalhammer-Reyero
patent: 5998383 (1999-12-01), Wright et al.
patent: 6189013 (2001-02-01), Maslyn et al.
patent: 6303297 (2001-10-01), Lincoln et al.
patent: 6308170 (2001-10-01), Balaban
patent: 2507901 (1970-11-01), None
patent: 0061761 (1982-10-01), None
patent: 2040943 (1980-09-01), None
patent: 5742632 (1986-03-01), None
patent: 61103824 (1986-05-01), None
patent: 8302276 (1983-07-01), None
Mackey et al., Biochemistry 16(20):4478-4482, 1977.
Sokja et al. Nucleic Acids Res, 5(2):385-401, 1978.
Roychoundhury et al., Nucleic Acids Res, 3(1):101-116, 1976.
Halloran et al. (J. of Immun. (1966), 96(3): 379-385).
Lehninger, Biochemistry [Published by Worth Publishers, Inc., 70 Fifth Ave., New York, New York, USA] (1970) pp. 638-639.
Dunn et al., Cell, vol. 12, pp. 23-36 (1977).
Hartman et al., Biopolymers, vol. 20, pp. 2635-2648 (1981).
Torrence et al.,J. Med. Chem.21:228-231 (1978).
Michelson,Biochem. Biophys., Acta91:1-13 (1964)
Cech et al.,Nucleic Acids Research2:2183-2192 (1979).
Bleackley et al.,Nucleic Acids Research2:683-690 (1975).
Roberts et al,J. Am. Chem. Soc.74:668-669 (1952).
Bauman et al.,Chromosoma84:1-18 (1982).
Bauman et al.,Exp. Cell Res.126:485-490 (1980).
Gerherd et al.,PNAS(USA)78:3755-3759 (1981).
Miller J.,Experiments in Molecular Genetics.Cold Spring Harbor Laboratory pp. 367-370 (1972).
Ueda et al.J. Carbohydr., Nucleosides, Nucleotides 5:261-271(1978).
Brunngraber et al.,J. Biol. Chem. 242:4834-4840 (1967).
Wilchek et al.,Biochemistry 6:247-252 (1967).
Monod et al.,J. Mol. Biol. 12:88-118 (1965).
Pastan et al.,Science 169:339-334 (1969).
Gilbert et al.,PNAS(USA)70:3581-3584 (1973).
Salam et al.,Carbohydrate Research 102:139-146 (1982)).
Pollack, S. E. and Auld, D. S., “Fluorescent Nucleotide Triphosphate Substrates for Snake Venom and Phosphodiesterase,” Analytical Biochemistry 127: 81-88 (1982).
Scopes, D. I. C., et al., “Defined Dimensional Changes in Enzyme Cofactors: Fluorescent “Stretched-Out” Analogs o Adenine Nucleotides,” Science 195: 296-298 (1977).
Onodera, M. and Yagi, K., “Synthesis of 2-(Dansylamino) Ethyl Triphosphate and its Properties as a Fluorescent Substrate of Heavy Meromyosin-ATPase,”Biochimica Et Biophysica 253: 254-265 (1971).
Yarbrough, L. R., “Synthesis and Properties of a New Flourescent Analog of ATP: Adenosine-5′-Triphosphoro-γ -1-(5-Sulfonic Acid) Napthylamidate,”Biochemical and Biophysical Research Communications 81(1): 35-41 1978).
Silver, M. S. and Fersht, A. R., “Direct Observation of Complexes Formed between recA Protein and a Fluorescent Single-Stranded Deoxyribonucleic Acid Derivative,”Biochemistry 21: 6066-6072 (Received Apr. 16, 1982).
Crane, L. J. and Miller, D. L., “Guanosine Triphosphate and Guanosine Diphosphate as Conformation-Determining Molecules. Differential Interaction of a Fluorescent Probe with the Guanosine Nucleotide Complexes of Bacterial Elongation Factor Tu,”Biochemistry 13(5): 933-938 (1974).
Hertz, H. S. and Zachau, H. G., “Kinetic Properties of Phenylalanyl-tRNA and Seryl-tRNA Synthetases for Normal Substrates and Flourescent Analogs,”Eur. Journal of Biochemistry 37: 203-213 (1973).
Wu, F. Y. H. and Wu, C. W., “Flourescent Affinity Labeling of Initiation Site on Ribonucleic Acid Polymerase ofEscherichia coli,” Biochemistry 13(12): 2562-2566 (1974).
McKenzie, R. L., et al., “Flourescent Conjugates of Natural and Biosynthetic Polynucleotides,”Biochemica Et Biophysica ACTA 277: 306-322 (1972).
Tournon, J., “Fluorescence Probing of Nucleic Acids: I. singly and doubly labeled dithymidine phosphate: fluorescence and energy transfer studies,”Nucleic Acids Research2(8): 1261-1273 (1975).
Erlanger, B. F., et al., “Use of Antibodies to Nucleosides and Nucleotides in Studies of Nucleic Acids in Cells,”Methods in Enzymology 60: 302-307 (1975).
Yarbrough, L. R., et al., “Synthesis and Properties of Flourescent Nucleotide Substrates for DNA-dependent RNA Polymerases,”The Journal of Biological Chemistry 254(23): 12069-12073 (1979).
Wintermeyer, W. and Zachau, H. G., “Flourescent Derivatives of Yeast tRNAPhe,”Eur. Journal of Biochem 98: 465-475 (1979).
Reines, S. A. and Schulman, L. H., “A New Method for Attachment of Fluorescent Probes to tRNA,” Methods in Enzymology 61: 146-157 (1979).
Barrio, J. R. et al., “Synthesis of Modified Nucleoside 3′, 5′-Bisphosphates and Their Incorporation into Oligoribonucleotides with T4 RNA Ligase,”Biochemistry 17(11): 2077-2081 (1978).
Koenig, P., et al., “Pyrene Derivatives as Fluorescent Probes of Conformation Near the 3′ Termini of Polyribonucleotides,”Biopolymers 16: 2231-2242 (1977).
Menzel, H. M., “On the Phe-tRNA induced binding of fluorescent oligonucleotides to the ribosomal decoding site,” Nucleic Acids Research 4(8): 2881-2892 (1977).
Leonard, N. J. and Tolman, G. L., “Fluorescent Nucleosides and Nucleotides,”Annals of the New York Academy of Sciences 255: 43-58 (1975).
Gavrilovskaya, I. N., et al., “Immunofluorescent Demonstration of Double-Stranded RNA and Virus Antigen in RNA Virus-Infected Cells,”Virology 62: 276-279 (1974).
Kukhavova, M. K., et al., “Peptidyl-tRNA With A Fluorescent Label: Ribosome Substrates in Peptide Bond Formation,” Molecular Biology Reports 1: 397-400 (1974).
Lynch, D. C. and Schimmel, P. R., “Effects of Abnormal Base Ionizations on Mg2 + Binding to Transfer Ribonucleic Acid as Studied by Fluorescent Probe,”Biochemisgry 13(9): 1852-1861 (1974).
Bergst
Engelhardt Dean
Kirtikar Dollie
Kline Stanley
Rabbani Elazar
Stavrianopoulos Janes G.
Bogdanos Natalie
Brusca John S.
Enzo Life Sciences, Inc. c/o Enzo Biochem, Inc.
Fedus Ronald C.
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