Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-02-17
1997-02-04
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, 536 2432, 536 2433, 935 8, 935 17, 935 78, C07H 2104, C12Q 170, C12P 1934
Patent
active
055996620
ABSTRACT:
The present invention provides improved primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from the pol gene of the human immunodeficiency virus type 1 (HIV-1). The invention also provides improved probes for the detection of the nucleic acid amplified using the primers of the invention. The primers and amplification methods of the invention enable the detection of HIV-1 from any of the known subtypes. The probes of the invention enable simple and rapid hybridization detection assays for detecting amplified HIV-1 nucleic acid.
REFERENCES:
Kwok et al., 1990, "Effects of Primer-Template Mismatches on the Polymerase Chain Reaction: Human Immunodeficiency Virus Type 1 Model Studies" Nucleic Acids Research 18(4):999-1005.
Coutlee et al., 1991, "The Polymerase Chain Reaction: A New Tool for the Understanding and Diagnosis of HIV-1 Infection at the Molecular Level" Molecular and Cellular Probes 5:241-259.
Jackson et al., 1991, "Non-Isotopic Polymerase Chain Reaction Methods for the Detection of HIV-1 in Ugandan Mothers and Infants" AIDS 5:1463-1467.
Chang et al., 1993, "Detection of Variability in Natural Populations of Viruses by Polymerase Chain Reaction" Methods in Enzymology 224:428-438.
Gurtler et al., Mar., 1994, "A New Subtype of Human Immunodeficiency Virus Type 1 (MVP-5180) from Cameroon" J. Virology 68(3):1581-1585.
Haesevelde et al., Mar., 1994, "Genomic Cloning and Complete Sequence Analysis of a Highly Divergent African Human Immunodeficiency Virus Isolate" J. Virology 68(3):1586-1596.
Loussert-Ajaka et al., Jun., 1994, "HIV-1/HIV-2 Seronegativity in HIV-1 Subtype O Infected Patients" Lancet 343:1393-1394.
Kwok et al., 1994, "A Guide to the Design and Use of Mismatched and Degenerate Primers" PCR Methods and Applications 3:S39-S47.
Fransen et al., 1994, "Design and Evaluation of New, Highly Sensitive and Specific Primers and Polymerase Reaction Detection of HIV-1 Infected Primary Lymphocytes" Molecular and Cellular Porbes 8:317-322.
Hoffmann-La Roche Inc.
Johnston George W.
Myers Carla J.
Petry Douglas A.
Sias Stacey R.
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