Oil bodies and associated proteins as affinity matrices

Details Oil bodies and associated proteins as affinity matrices Oil bodies and associated proteins as affinity matrices

1996-12-16

1999-01-05

Grimes, Eric

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435262, 435270, 435272, 435277, C07K 114, C07K 122, C12S 314, C12S 1300

Patent

active

058564527

ABSTRACT:
A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.

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patent: 5538946 (1996-07-01), Crause et al.
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Pireset al. Biotechnol. Prog. 12:290-301, 1996.
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Van Rooijen and Moloney, Plant Physiol 109:1353-1361, 1995.
von Rooijen, Gijs J.H., "Molecular Biology of Oil Body Proteins in the Brassicaceae: Structure, Function and Biotechnological Applications," Ph.D. thesis, pp. 177-191, 1993.

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