Octopine T-DNA structural genes

Details Octopine T-DNA structural genes Octopine T-DNA structural genes

1995-06-02

2000-07-18

Fox, David T.

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

Plant cell or cell line, per se, contains exogenous or...

435 691, 4352522, 4352523, 4353201, 435468, 435469, 536 236, 536 237, 536 241, C12N 504, C12N 1529, C12N 1531, C12N 1584

Patent

active

060906278

ABSTRACT:
The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

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