Octopine T-DNA promoters

Details Octopine T-DNA promoters Octopine T-DNA promoters

1993-07-13

1995-06-27

Fox, David T.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 435 701, 4351723, 4352404, 4352523, 4353201, C07H 2104, C12N 500, C12N 1500, C12P 2104

Patent

active

054281474

ABSTRACT:
The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

REFERENCES:
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Lippincott et al. 1978. Science 199: 1075-1078.
Krens et al. 1982. Nature 296: 72-74.
Hernalsteens et al. 1984. EMBO J 3(13): 3039-3041.

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