Octanucleotide restriction endonuclease, Srf I, and method for p

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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C21N 922

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active

060543078

ABSTRACT:
This invention provides restriction endonuclease Srf I, which is capable of recognizing the eight nucleotide base palindromic sequence shown below on a double stranded DNA molecule and cleaving the DNA chain at the asterisk-marked position resulting in blunt ends

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Kotani, et al. Nucleic Acids Res., 18:5637-5640 (1990).
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Nelson, et al. Nucleic Acids Res., 18:2061-2064 (1990).
Roberts, Nucleic Acids Res., 18:2331-2365 (1990).
Roberts, et al. Nucleic Acids Res., 20:2167-2180 (1992).
Tautz, et al. Nucleic Acid Res., 18:3087 (1990).
Simcox, et al. Gene, 109: 121-123 (1991).
Kessler, et al. Gene, 92: 241-245 (1990).

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