Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...
Reexamination Certificate
2011-07-12
2011-07-12
Martinell, James (Department: 1634)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Animal cell, per se, expressing immunoglobulin, antibody, or...
C435S395000
Reexamination Certificate
active
07977093
ABSTRACT:
An in vitro method for the obtention of a food- or auto-antigen specific Tr1 cell population from a leukocyte or a PBMC population, includes stimulating the PBMC or leukocyte population with the food- or auto-antigen, and recovering the food- or auto-antigen specific Tr1 cell population from the stimulated cell population. Preferably, the PBMC or leukocyte population is re-stimulated at least once with the same antigen after step (1), in the presence of IL-2 and at least one interleukin selected from the group consisting of IL-4 and IL-13. The in vitro method may further include a third step of expanding the recovered antigen-specific Tr1 cell population, advantageously by contacting them with feeder cells capable of expressing factors necessary for the expansion. Preferably, the feeder cells are recombinant insect feeder cells.
REFERENCES:
patent: 2004/0191235 (2004-09-01), Groux et al.
patent: WO 02/092793 (2002-11-01), None
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Levings et al, J. Immunol. 166 (9), 5530 (2001).
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Brun Valérie
Cottrez Françoise
Foussat Arnaud
Groux Hervé
INSERM (Institut National de la Sante et de la Recherche Medical
Martinell James
TxCell
Young & Thompson
LandOfFree
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