Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Process involving micro-organisms of different genera in the...
Reexamination Certificate
2005-11-22
2005-11-22
Lilling, Herbert J. (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Process involving micro-organisms of different genera in the...
C435S037000, C435S039000, C435S252310, C435S252400, C435S252500
Reexamination Certificate
active
06967087
ABSTRACT:
The present invention relates to a novel obligately symbiotic thermophileSymbiobacterium toebiiSC-1(Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced bySymbiobacterium toebiiSC-1. The present invention also relates to a pure culture method ofSymbiobacterium toebiiSC-1, a method of growth measurement forSymbiobacterium toebuSC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains ofSymbiobacterium toebiiSC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced bySymbiobacterium toebiiSC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
REFERENCES:
patent: 5056779 (1993-03-01), None
patent: 11042097 (1999-02-01), None
Seung-Goo Lee et al, “Thermostable Tyrosine Phenol-Lyase of Symbiobacterium sp. SC-1: Gene Cloning, Sequence Determination, and Overproduction inEscherichia coli,” Protein Expression and Purification 11:263-270 (1997).
Michiyo Ohno et al., “Establishing the Independent Culture of a Strictly Symbiotic Bacterium Symbiobacterium thermophilum from Its Supporting Bacillus Strain,” Biosci. Biotechnol. Biochem., 63(6):1083-1090 (1999).
Bae Jin-Woo
Hong Seung-Pyo
Jeon Che Ok
Kang Dae-Joong
Kim Chul-Joong
Gates & Cooper LLP
Korea Research Institute of Bioscience and Biotechnology
Lilling Herbert J.
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