Objective biochemical method for determining fertilization poten

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435 4, 435 29, C12Q 150

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active

049450441

ABSTRACT:
A method for testing sperm quality comprising obtaining a sperm sample; detecting CK enzyme from the sperm sample; measuring a first CK enzyme concentration for CKX isoforms of the CK enzyme; and determining a sperm quality parameter based upon the first CK enzyme concentration. Preferably a second CK enzyme concentration is measured for CKB isoforms of the CK enzyme and is also used as a basis for determining the sperm quality parameter. Measurements of the levels of CKX and CKB isoforms by electrophoresis and fluorescence techniques are used to establish the first and second CK enzyme concentrations. The sperm quality parameter is proportional to the ratio of the CKX isoform level to the sum of the CKX and CKB isoform levels. Sperm which meets a predetermined minimum sperm quality level is selected for use in vivo or in vitro fertilization attempts on eggs.

REFERENCES:
Huszar et al.--Gamete Research, vol. 19 (1988) pp. 67-75.
Huszar et al.--Annals N.Y. Acad. Sci.--Vol. 513 (1987), pp. 602-605.
Wallimann et al.--Chem. Abst. Vol. 104 (1986), page 183729c.
Huszar et al.--Chem. Abst. Vol. 109 (1988), p. 71522b.
Creatine Kinase Activity in Sperm: A possible New Diagnostic Tool of Sperm Function--Gabor Huszar, Lynne Vigue, Society for Gynecologic Investigation (1986).
CPK Activity Reliably Predicts Sperm Quality in Menn--Gabor Huszar, Lynne Vigue, Society for Gynecologic Investigation (1987).
Serial Sperm CPK Measurements in Oligospermic and Normospermic Men--Gabor Huszar, Lynne Vigue, 5th World Congress of in Vitro Fertilization and Embryo Transfer (1987).
Sperm Creatine Phosphokinase (CPK) Activity is a Predictor of Fertilizing Capacity in Oligospermic Men--Gabor Huszar, Lynne Vigue, American Fertility Society (1987).
Enhancement of Human Sperm Motility and Velocity in Vitro: Effects of Calcium and Creatine Phosphate, Fertility and Sterility--Fakih, MacLusky, DeCherney, Wallman and Huszar, Fertility and Sterility, vol. 46, No. 5, pp. 938-944, (Nov. 1986).
Ratio of B-Type and M-Type Sperm CPK Isoforms Correlates with CPK Activity and Sperm Quality in Oligosphermic Specimens--Huszar, Vigue, Quevedo, Vigue American Fertility Society 1988.
Increased Cellular CPK Concentrations Cause the Elevated Sperm CPK Activities in Oligospermic Specimens--Huszar, Vigue, Society for Gynecologic Investigation (1988).
Directions for Corning Electrophoresis Cardiotrac-CK Creatine Kinase Isoenzyme Substrate Set (Date unknown).
Directions for Helena Laboratories Titan Gel Iso-Dot CK Isoenzyme Procedure (Date unknown).
Beckman Paragon Electrophoresis System Creatine Kinase Isoenzyme Electrophoresis Kit Directions (Date unknown).

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