Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-04-04
1996-09-24
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352542, 4353201, 536 241, C12N 115, C12N 1511, C12N 1581, C12P 2106
Patent
active
055590101
ABSTRACT:
The invention provides methods and compositions for expressing a recombinant gene in eukaryotic cells, especially fungi, preferably yeast. The invention provides transcriptional regulating elements having a novel nucleotide sequence which are capable of trancriptionally regulating the expression of a cis joined gene, typically in response to the availability of certain nutrients to the host cell. Preferred regulatory elements are responsive to nutrient depletion, particulary glucose, ethanol, phosphate or a nitrogen source. Nucleic acid constructs comprising such regulatory elements operably linked to recombinant genes, cells comprising such regulatory elements, and methods of producing recombinant protein in such cells are also provided. The invention discloses regulatory elements which are induced through the ras gene product. Accordingly, the disclosed expression systems also provide a convenient marker for ras gene function. Finally, the invention also provides methods and compositions for the diagnosis and treatment of fungal infection. In particular, the invention provides gp37-derived peptides encoded by YGP1 and gp37- selective binding agents, such as antibodies.
REFERENCES:
Law and Segall, "The SPS100 Gene of Saccharomyces cerevisiae Is Activated Late in the Sporulation Process and Contributes to Spore Wall Maturation", Mol. and Cell. Biol. 8(2):912-922 (1988).
Mittenbuhler and Holzer, "Characterization of Different Forms of Yeast Acid Trehalase in the Secretory Pathway", Arch. Microbiol. 155:217-220 (1991).
Mittenbuhler and Holzer, "Purification and Characterization of Acid Trehalase from the Yeast suc2 Mutuant", J. of Biol. Chem. 263(17):8537-8543 (1988).
Fincham, Microbiological Reviews, Mar. 1989, pp. 148-170.
Latchman, Eukaryotic Transcription Factors, Mar. 30, 1992, Academic Press Inc., San Diego CA, pp. 87-89.
Destruelle et al, Molecular and Cellular Biology, Mar. 25, 1994, pp. 2740-2754.
Destruelle Monika
Holzer Helmut
Klionsky Daniel J.
Elliott George C.
McKelvey Terry A.
Osman Richard Aron
The Regents of the University of California
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