Nutrient mixtures for the bioremediation of polluted soils and w

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

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210604, C02F 300, C12N 120

Patent

active

056353928

DESCRIPTION:

BRIEF SUMMARY
BACKGROUND OF THE INVENTION

Controlling pollution caused by hydrocarbon compounds, for example crude oil pollution, in soils and waters by bioremediation is acquiring increasing significance. Major advantages are afforded by the inexpensive in situ processes where no space is required for waste disposal. Microorganisms which consume hydrocarbon compounds are valuable tools in the context of this technology, even under comparatively unfavorable basic conditions, providing their enrichment and/or their growth at the point of pollution can be sufficiently stimulated. From the more recent literature, reference is made to the articles in Chemische Industrie 5/91, 10 to 12 "Hunger for Crude Oil" and in Erdol und Kohle-Erdgas, 44, April 1991, 197 to 200, Th. Hopner et al. "Die Olkatastrophe im Persisch-Arabischen Golf (The Oil Catastrophe in the Persian/Arabian Gulf)" and the extensive literature cited therein.
The working principle of bioremediation is based on optimal promotion of the growth of the pollution-consuming microorganism populations. Two important aids are of paramount significance in this regard: firstly, the supply of growth-promoting elements which are generally not available in sufficient concentrations in the polluted area. These growth-promoting elements are primarily inorganic and/or organic compounds of nitrogen and phosphorus which are made available as nutrient concentrates for stimulation and as growth aids for the accelerated growth of the microorganisms consuming hydrocarbon compounds. Secondly, preformed concentrates of suitable hydrocarbon-consuming microorganisms are frequently used, in particular to accelerate biological degradation in the initial phases, and may be applied to the polluted areas, for example at weekly intervals. However, depending on the particular situation, more particularly the previous individual history of the polluted areas to be treated, it may even be assumed that the use of microorganism concentrates is not necessary. In general, this will always be the case when the natural process of biological degradation has already resulted in the development of sufficient concentrations of microorganism strains, cf. in this connection for example the 2nd of the literature references cited at the beginning.
Hitherto unpublished German patent application DE-P 41 31 714.9 proposes an improved nutrient mixture for the bioremediation of polluted soils and waters. The mixture described in this document is in the form of a storable solution or emulsion and contains P- and N-sources in a liquid mixed phase of biocompatible water-soluble carrier components based on glycerol and biocompatible oil-soluble carrier components based on glycerol esters. Although the application in question proposes a solution in which only substances which are readily biodegradable are introduced into the contaminated soil sample, another C-source is introduced on a significant scale in the form of the glycerol esters. Under unfavorable growth conditions, the presence of these components can promote the growth of microorganisms which are unsuitable for degrading pollutants so that they proliferate to the detriment of the degrading microorganisms.


DESCRIPTION OF THE INVENTION

Against this background, the problem addressed by the present invention was to provide a nutrient mixture which would have an emulsifying effect and which would contain P- and N-yielding substances, but would be made up of very few pure C-compounds.
Another problem addressed by the invention was to prepare this nutrient mixture in such a way that it would leave very few residues behind in the soil sample to be treated. A further problem addressed by the invention was to provide a nutrient mixture which would prefer the pollutant degrading microorganisms to other microorganisms.
In a first embodiment, therefore, the present invention relates to a nutrient concentrate for stimulation and as a growth aid for the accelerated growth of hydrocarbon-consuming microorganisms for their use in the biological degradation of organic comp

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Water Environment Research vol. 64, No. 2, Apr. 1992, Alexandria, VA, pp. 163-169, D.M. Falatko et al "Effects of Biologically Produced Surfactants on the Mobility and Biodegradatin of Petroleum Hydrocarbons" see page 167, column 2 -page 168.
Chemische Industrie May 1991, 10 to 12 "Hunger for Crude Oil".
Erdol und Kohle -Erdgas, 44, Apr. 1991, 197 to 200, Th. Hopner et al. "Die Olkatastrophe im Persisch-Arabischen Golf".
"Bioremediation of Hazardous Waste" in Biofuture, Sep. 1990, 24 to 35.

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