Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-10-31
1999-01-19
Houtteman, Scott W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
4353201, C07H 2104
Patent
active
058614982
ABSTRACT:
A substantially pure protein, immunophilin FKBP46, is disclosed. An isolated nucleic acid molecule that comprises a nucleic acid sequence that encodes immunophillin FKBP46, is disclosed. An isolated nucleic acid molecule consisting of a nucleic acid sequence that encodes immunophillin FKBP46, or a fragment thereof having at least 10 nucleotides is disclosed. Recombinant expression vector comprising a nucleic acid sequence that encodes immunophillin FKBP46 and host cells comprising the recombinant expression vector are disclosed. Oligonucleotide molecule comprising a nucleotide sequence complimentary to a nucleic acid sequence that encodes immunophillin FKBP46 of at least 5 nucleotides are disclosed. Antibodies that binds to an epitope on FKBP46 are disclosed. Methods of identifying immunosuppressive drugs comprising the steps of contacting FKBP46 or a homologous protein derived from yeast with a test compound and determining whether the protein binds to the test compound are disclosed.
REFERENCES:
patent: 4736866 (1988-04-01), Leder et al.
patent: 4873191 (1989-10-01), Wagner et al.
Database Medline on STN; US National Library of Medicine, (Bethesda, MD, USA), No. 95074110, Alnemri et al., J. Bio. Chem., 269(49):30828-34, Sep. 1994.
Alnemri, et al., "Overexpressed full-length human BCL2 extends the survival of baculovirus-infected Sf9 insect cells", Proc. Natl. Acad. Sci. USA, 1992, 89, 7295-7299.
Alnemri, E.S. and Litwack, G., "The Steroid Binding Domain Influences Intracellular Solubility of the Baculovirus Overexpressed Glucocorticoid and Mineralocorticoid Receptors", Biochem., 1993, 32, 5387-5393.
Alnemri, et al., "Overexpression, characterization, and purification of a recombinant mouse immunophilin FKBP-52 and identification of an associated phosphoprotein", Proc. Natl. Acad. Sci. USA, 1993, 90, 6839-6843.
Alnemri, et al., "Characterization and Purification of a Functional Rat Glucocorticoid Receptor Overexpressed in a Baculovirus System", J. Biol. Chem., 1991, 266, 3925-3936.
Baskaran, B. and Rao, M.R.S., "Mammalian Spermatid Specific Protein, TP2, is a Zinc Metalloprotein with Two Finger Motifs", Biochem. Biophys. Res. Commun., 1991, 179, 1491-1499.
Cole, K.D. and Kistler, S., "Nuclear Transition Protein 2 (TP2) of Mammalian Spermatids Has a Very Basic Carboxyl Terminal Domain", Biochem. Biophys. Res. Commun., 1987, 147, 437-442.
Galat, et al., "A Rapamycin-Selective 25-kDa Immunophilin", Biochem., 1992, 31, 2427-2434.
Gething, M.J. and Sambrook, J., "Protein folding in the cell", Nature, 1992, 355, 33-45.
Heitman, et al., "Targets for Cell Cycle Arrest by the Immunosuppressant Rapamycin in Yeast", Science, 1991, 253, 905-909.
Heitman, et al., "Proline Isomerases at the Crossroads of Protein Folding, Signal Transduction and Immunosuppression", New Biol., 1992, 4, 448-460.
Harrison, R.K. and Stein, R.L., "Substrate Specificities of the Peptidyl Prolyl Cis-Trans Isomerase Activities of Cycophilin and FK-506 Binding Protein: Evidence for the Existence of a Family of Distinct Enzymes", Biochem., 1990, 29, 3813-3816.
Jin, Y.J. and Burakoff, S.J., "The 25-kDa FK506-binding protein is localized in the nucleus and associates with casein kinase II and nucleolin", Proc. Natl. Acad. Sci. USA, 1993, 90, 7769-7773.
Johnson, P.F. and McKnight, S.L., "Eukaryotic Transcriptional Regulatory Proteins", Annu. Rev. Biochem., 1989, 58, 799-839.
Kalderon, et al., "Sequence requirements for nuclear location of simian virus 40 large-T antigen", Nature, 1984, 311, 33-38.
Koltin, et al., "Rapamycin Sensitivity in Saccharomyces cerevisiaeIs Mediated by a Peptidyl-Prolyl cis-trans Isomerase Related to Human FK506-Binding Protein", Mol. Cell. Biol., 1991, 11, 1718-1723.
Liu, et al., "Calcineurin is a Common Target of Cyclophilin-Cyclosporin A and FKBP-FK506 Complexes", Cell, 1991, 66, 807-815.
Liu, et al., "Inhibition of T Cell Signaling by Immunophilin-Ligand Complexes Correlates with Loss of Calcineurin Phosphatase Activity", Biochem., 1992, 31, 3896-3901.
Maki, et al., "Complementary DNA encoding the human T-cell FK506-binding protein, a peptidylprolyl cis-trans isomerase distinct from cyclophilin", Proc. Natl. Acad. Sci. USA, 1990, 87, 5440-5443.
Murre, et al., "A New DNA Binding and Dimerization Motif in Immunoglobulin Enhancer Binding, daughterless, MyoD, and myc Proteins", Cell, 1989, 56, 777-783.
Park, et al., "PPIase Catalysis by Human FK506-binding Protein Proceeds Through a Conformational Twist Mechanism", J. Biol. Chem., 1992, 267, 3316-3324.
Peattie, et al., "Expression and characterization of human FKBP52, an immunophilin that associates with the 90-kDa heat shock protein and is a component of steroid receptor complexes", Proc. Natl. Acad. Sci. USA, 1992, 89, 10974-10978.
Robbins, et al., "Two Interdependent Basic Domains in Nucleoplasmin Nuclear Targeting Sequence: Identification of a Class of Bipartite Nuclear Targeting Sequence", Cell, 1991, 64, 615-623.
Schreiber, "Chemistry and Biology of the Immunophilins and Their Immunosuppressive Ligands", Science, 1991, 251, 283-287.
Schreiber, "Immunophilin-Sensitive Protein Phosphatase Action in Cell Signaling Pathways", Cell, 1992, 70, 365-368.
Schreiber, S.L. and Crabtree, G.R., "The mechanism of action of cyclosporin A and FK506", Immunol. Today, 1992, 13, 136-142.
Shirakawa, et. al., "Primary Structure of Non-Histone Chromosomal Protein HMG2 Revealed by the Nucleotide Sequence", Biochem., 1990, 29, 4419-4423.
Siekierka, et. al., "The Cytosolic-binding Protein for the Immunosuppressant FK-506 Is Both a Ubiquitous and Highly Conserved Peptidyl-Prolyl Cis-Trans Isomerase", J. Biol. Chem., 1990, 265, 21011-21015.
Sigal, N.H. and Dumont, F.J., "Cyclosporin A, FK-506, and Rapamycin: Pharmacologic Probes of Lymphocyte Signal Transduction", Annu. Rev. Immunol., 1992, 10, 519-560.
Stamnes, et al., "The Cyclophilin Homolog ninaA is a Tissue-Specific Integral Membrane Protein Required for the Proper Synthesis of a Subset of Drosophila Rhodopsins", Cell, 1991, 65, 219-227.
Standaert, et al., "Molecular cloning and overexpression of the human FK506-binding protein FKBP", Nature, 1990, 346, 671-674.
Tai, et al., "Association of a 50-Kilodalton Immunophilin with the Glucocorticoid Receptor Complex", Science, 1992, 256, 1315-1318.
Tai, et al., "P59 (FK506 Binding Protein 59) Interaction with Heat Shock Proteins is Highly Conserved and May Involve Proteins Other Than Steroid Receptors", Biochem., 1993, 32, 8842-8847.
Van Duyne, et al., "Atomic Structure of FKBP-FK506, an Immunophilin-Immunosuppressant Complex", Science, 1991, 252, 839-842.
Alnemri Emad S.
Fernandes-Alnemri Teresa
Litwack Gerald
Houtteman Scott W.
Thomas Jefferson University
LandOfFree
Nucleotides encoding immunophilin FKBP46 and fragments thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleotides encoding immunophilin FKBP46 and fragments thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleotides encoding immunophilin FKBP46 and fragments thereof will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1247633