Nucleotide sequences which hybridize specifically with a Campylo

Details Nucleotide sequences which hybridize specifically with a Campylo Nucleotide sequences which hybridize specifically with a Campylo

1994-09-29

1997-11-25

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, C12Q 168, C07H 2102, C07H 2104

Patent

active

056911386

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to a nucleic sequence specific for Campylobacter jejuni as well as to the applications of this sequence as nucleotide probe specific for the detection of Campylobacter jejuni sequences or fragments of this sequence as nucleotide primers for the amplification of Campylobacter jejuni DNA or RNA in a biological sample.
Campylobacter infections are widespread throughout the World, affecting both men and wild or domestic animals.
Although discovered at the beginning of the twentieth century, the bacteria currently called Campylobacter were for long ignored because their characteristics made their identification and their culture difficult. First isolated from the Ovidae and the Bovidae and called Vibrio fetus then, later, Campylobacter fetus, it was only from 1946 that the first cases of human campylobacterioses were described, but it was only from 1972, when selective media for Campylobacter started to be developed, that the importance of Campylobacter infections was able to be proved and recognized.
Since the naming of the type species Campylobacter fetus, about twelve other species and subspecies have been discovered, the exact number varying according to the authors and taxonomic methods, who often propose new classification criteria. Among these species, those most frequently encountered in human and/or animal pathology are Campylobacter jejuni, Campylobacter coli and Campylobacter fetus.
Currently, Campylobacter jejuni is considered as one of the most frequent causes of infectious diarrhoea in man.
The "national network for monitoring Campylobacter infections", set up in France in 1986, publishes each year an assessment setting out the principal epidemiological and clinical data for the cases reported. For the years 1988, 1989 and 1990 for example, it seems that the species most frequently implicated in these infections (from 60 to 75% of the cases analysed) was C. jejuni.
In the human species, the major symptom of intestinal C. jejuni infection is diarrhoea which, in the most serious cases, can cause severe water loss, which can be particularly dangerous for children and infants, who are very sensitive to dehydration. However, C. jejuni enteriris often remains without complications and the diarrhoeas can even cease spontaneously after one week. However, coprocultures may remain positive after a few weeks or even months, and, in 5 to 10% of cases, relapses can occur. Vigorous treatment and monitoring are therefore necessary, especially for immunosuppressed individuals or individuals having serious diseases (AIDS, cirrhosis of the liver, cancer, leukaemia and the like), in whom Campylobacter can behave like opportunistic bacteria.
Other consequences of C. jejuni infections have also been described, although they are more rare or exceptional: mesenteric adenitis, cholecystitis, urinary infections, meningitis, septicaemias, erythema nodosum or Guillain-Barre syndrome and the like.
In animals, Campylobacter usually lives as commensals in the digestive tube of numerous species: bovines, ovines, pigs, poultry, wild birds, dogs and cats. These animals, diseased or healthy carriers, constitute a big reservoir of microbes, and therefore a high risk of contamination. In the case of obvious infections, in bovines and ovines, C. jejuni is known, since the first description in 1931, as being the cause of "cattle dysentery", which may have as a consequence, besides the effect on the cattle, the transmission to man through the spread of the microbes in the surroundings of the animals (land, water). Even for asymptomatic animals, "healthy carriers", the transmission to man can occur: either by direct contact with these animals or their excrement, or by consumption of contaminated foods or water (meats contaminated during their preparation and poorly cooked, unpasteurized milk, polluted water and the like).
From a prevention perspective, it is therefore important, both in man and in animals, to be able to identify the pathogen C. jejuni as early as possible, so as to prevent, by adequate meas

REFERENCES:
Sambrook et al., "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory, NY (1989).
Choi et al. Nucleic Acids Research 16 (15) :7732 (1988).
Oyofo et al., J. of Clinical Microbiology 30 (10) : 2613-2619 (1992).
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Jablonski et al., Nucleic Acids Research 14 (15): 6115-6128 (1986).
Chevrier et al., J. of Clinical Microbiology 27 (2) : 321-326 (1989).
Korolik et al., J. of Gemneral Microbiology 134 :521-529 (1988).

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