Nucleotide sequences which code for the menE gene

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

Reexamination Certificate

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C435S069100, C435S252300, C435S252320, C435S320100, C530S350000, C536S023200

Reexamination Certificate

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06946271

ABSTRACT:
The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting ofa) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2,c) polynucleotide which is complementary to the polynucleotides of a) or b), andd) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

REFERENCES:
patent: 1 108 790 (2001-06-01), None
patent: WO 0 100 843 (2001-01-01), None
Attwood et al. Which craft is best in bioinformatics? Comput. Chem. 2001, vol. 25(4), pp. 329-339.
Ponting, C.P. Issues in predicting protein function from sequence. Brief. Bioinform. Mar. 2001, vol. 2(1), pp. 19-29.
Du, L. Accession AD000004. Dec. 3, 1996.
Smith, D.R. Accession U15187. Mar. 9, 1995.
Sieweke HJ et al. (1991) “o-Succinylbenzoate: coenzyme A ligase, an enzyme involved in menaquinone (vitamin K2) biosynthesis, displays broad specificity,”Z Naturforsch[C] 46: 585-90 (abstract).
Kwon O et al. (1996) “Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and properties of o-succinylbenzoyl-coenzyme A synthetase fromEscherichia coli,” J Bacteriol178: 6778-81.
Meganathan R et al. (1979) “Menaquinone (vitamin K2) biosynthesis: conversion of o-succinylbenzoic acid to 1,4-dihydroxy-2-naphthoic acid byMycobacterium phleienzymes,”J Bacteriol140: 92-8.
Kramer, “Genetic and physiological approaches for the production of amino-acids”, Journal of Biotechnology, vol. 45, No. 1. p. 1-21.
Eikmanns et al., “Molecular aspects of lysine, threonine, and isoleucine biosynthesis inCorynebacterium glutamicum”, Antonie Van Leeuwenhoek, Dordrecht, vol. 64, No. 2, p. 145-163.
Database EMBL Online, Accession No. XP002184747.
Database EMBL Online, Accession No. XP002186152.
Database EMBL Online, Accession No. XP002184749.

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