Nucleotide sequences which code for the cstA gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S115000, C435S320100, C435S252300, C435S252320, C536S023100

Reexamination Certificate

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06972190

ABSTRACT:
The invention relates to an isolated polynucleotide fromCorynebacterium glutamicumhaving a polynucleotide sequence which codes for the carbon starvation protein A (cstA) gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in enhanced form and a vector which carries at least the cstA gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

REFERENCES:
patent: 6822084 (2004-11-01), Pompejus et al.
patent: 2002/0197605 (2002-12-01), Nakagawa et al.
patent: 1 108 790 (2001-06-01), None
patent: WO 99/18228 (1999-04-01), None
patent: WO 01/00804 (2001-01-01), None
Cole et al. Deciphering the biology ofMycobacterium tuberculosisfrom the complete genome sequence. Nature (1998) 393:537-544.
Dubey et al. CsrA regulates translation of theEscherichia colicarbon starvation gene, cstA, by blocking ribosome access to the cstA transcript. J. Bacteriol. (2003) 185(15):4450-4460.
GenBank Accession No. BX927149.Corynebacterium glutamicumATCC 13032, IS fingerprint type 4-5, complete genome segment 2/10, Jan. 21, 2004.
Bernhard J. Eikmanns, et al., “Molecular aspects of lysine, threonine, and isoleucine biosynthesis inCorynebacterium glutamicum,” Antonie Van Leeuwenhoek, vol. 64, 1993, pp. 145-163.
Nucleotide Sequence ID 4227 and Database EBI, Hinxton, UK; Acession No.: AX124311, May 10, 2001.
Nucleotide Sequence ID 7061 and Database EBI, Hinxton, UK; Acession No.: AX127145, May 10, 2001.
Nucleotide Sequence of Cole, et al. and Database EBI, Hinxton, UK; Acession No.: 283866, Jan. 15, 1997.
GenBank Accession No. P15078, Nov., 1997.

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