Nucleotide sequences which code for the citB gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S252320, C435S252330, C435S320100, C435S069100, C435S325000, C435S232000, C435S004000, C536S023100, C536S023200

Reexamination Certificate

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06939694

ABSTRACT:
The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the citB gene, and a host-vector system having a coryneform host bacterium in which the citB gene is present in attenuated form and a vector which carries at least the citB gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

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Todo et al., GenEMBL accession No. AF265258, May 30, 2000.
Ikeda et al. , Appl. Environ. Microbiol., 65:2494-2502, 1999.
Sambrook et al. , Molecular Cloning, Second Edition, CSHL Press, 1989.
Seffernick et al. , J. Bacteriol. 183(8):2405-2410, 2001.
Michael Bott, et al., “Regulation of anaerobic citrate metabolism in Klebsiella pneumoniae”, Molecular Microbiology (1995) 18(3), pp. 533-546.
Paul Golby, et al., “Identification and Characterization of a Two-Component sensor-Kinase and Response-Regulator system (DcnS-DcnR) Controlling gene Expression in Response to C4-Dicarboxylates inEscherichia coli”, Journal of Bacteriology, pp. 1238-1248 (Feb. 1999).

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