Nucleotide sequences permitting the replication and maintenance

Details Nucleotide sequences permitting the replication and maintenance Nucleotide sequences permitting the replication and maintenance

1994-07-26

1997-01-28

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 9141, 4351723, 4353201, 435348, 536 2372, 536 241, C12Q 170, C12N 1564

Patent

active

055976891

ABSTRACT:
The subject of the invention is the use of a DNA fragment comprising a sequence from the 3' end of the AcNPV baculovirus IE1 gene, optionally combined with a DNA fragment comprising a sequence situated upstream of the promoter of the IE1 gene, for producing vectors permitting the replication and maintenance of genetic information in animal cells.
The invention also encompasses the episomal vectors obtained, as well as the transformed cells comprising the vectors.

REFERENCES:
patent: 5004687 (1991-04-01), Miller
Pennoch et al., "Strong and Regulated Expression of Escherichia coli .beta.-Galactosides in Insect Cells with a Baculovirus Vector", Mol. Cell. Biol., vol. 4, No. 3, Mar. 1984, pp. 399-406.
Hooft, B. J. L. et al., "Detection of Sequences in Autographa californica Nuclear Polyhedrosis Virus DNA, that Act as Autonomous Replicating Sequences (ARS) in Yeast," Archives of Virology, vol. 88, 1986, pp. 279-284.
Guarino, L. A. and Summers, M. D., "Nucleotide Sequence and Temporal Expression of a Baculovirus Regulatory Gene," Journal of Virology, vol. 61, No. 7, Jul. 1987, pp. 2091-2099.
Lee, H. Y. et al., "Identification of bent DNA and ARS fragments in the genome of Choristoneura fumiferana nuclear polyhedrosis virus," Virus Research, vol. 24, 1992, pp. 249-264.
Leisy, D. and Rohrmann, G. F., "Characterization of the Replication of Plasmids Containing hr Sequences in Baculovirus-Infected Spodoptera frugiperda Cells," Virology, vol. 196, No. 2, Oct. 1993, pp. 772-730.

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