Nucleotide sequences of a T. pallidum rare outer membrane protei

Details Nucleotide sequences of a T. pallidum rare outer membrane protei Nucleotide sequences of a T. pallidum rare outer membrane protei

1997-04-23

1998-10-13

Myers, Carl J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352523, 4353201, 536 237, 536 2432, 536 2433, 935 8, 935 11, 935 66, C07H 2104, C12N 1500, C12N 1563, C12N 121

Patent

active

058210857

ABSTRACT:
Antigenic rare outer membrane proteins of Spirochaetaceae are obtained from organisms extracted from infected tissue by a novel process of isolation utilizing a discontinuous Ficoll gradient separation, release of outer membrane in a low isotonic and low pH buffer and identification of outer membrane by use of a lipid soluble dye. Four antigenic rare outer membrane proteins of T. pallidum subsp. pallidum useful in diagnosis and prophylaxis of syphilis are provided. Also provided is the amino acid sequence of a rare outer membrane protein of T. pailidum subsp. pallidum and the nucleotide sequence encoding it.

REFERENCES:
Norris et al, Microbiological Reviews (1993) 57:750-779.
Blanco et al, 1991, Molecular Microbiology 5(10) 2405-2415.

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