Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-11-28
2006-11-28
Kaushal, Sumesh (Department: 1633)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S252300, C435S252320, C435S471000, C536S023100, C536S023200
Reexamination Certificate
active
07141388
ABSTRACT:
The invention relates to isolated polynucleotides fromCorynebacterium glutamicumwhich are useful in the regulation of gene expression. In particular, the invention relates to isolated polynucleotides comprisingC.glutamicumpromoters which may be used to regulate, i.e., either increase or decrease, gene expression. In certain embodiments, isolated promoter sequences of the present invention regulate gene expression through the use of exogenous or endogenous induction. The invention further provides recombinant vectors and recombinant cells comprising isolated polynucleotides of the present invention, preferably in operable association with heterologous genes. Also provided are methods of regulating bacterial gene expression comprising growth of a recombinant cell of the present invention. In particular, the present invention provides methods to regulate genes involved in amino acid production comprising growth of a recombinant cell of the present invention. In certain embodiments, the present invention provides methods of regulating gene expression in bacteria, particularlyCorynebacteriumspecies, especially of the genusCorynebacterium, comprising fermentation growth of a recombinant cell of the present invention, where metabolite concentrations, temperature, or oxygen levels are manipulated to regulate gene expression.
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Crafton Corey M.
Rayapati P. John
Archer-Daniels-Midland Company
Cochenour Craig G.
Kaushal Sumesh
Stewart III Duane A.
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