Nucleotide sequences for encoding of the lysR2-gene

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S320100, C435S069100, C435S252320

Reexamination Certificate

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07078502

ABSTRACT:
Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting ofa) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2,c) polynucleotide which is complementary to the polynucleotides of a) or b), andd) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

REFERENCES:
patent: 1 108 790 (2001-06-01), None
patent: WO 01/00804 (2001-01-01), None
Josef Cremer, et al., Applied and Environmental Microbiology, vol. 57, pp. 1746-1752, “Control of the Lysine Biosynthesis Sequence inCorynebacterium Glutamicumas Analyzed by Overexpression of the Individual Corresponding Genes”, Jun. 1991.
L. Eggeling, et al., Appl Microbiol Biotechnol, vol. 49, pp. 24-30, “Improved L-Lysine Yield withCorynebacterium Glutamicum: Use ofDapaResulting in Increased Flux Combined with Growth Limitation”, 1998.
Bernhard J. Eikmanns, et al., Antonie van Leeuwenhock, vol. 64, pp. 145-163, “Molecular Aspects of Lysine, Threonine, and Isoleucine Biosynthesis inCorynebacterium Glutamicum”, 1993.
C. Fernández-González, et al., Appl Microbiol Biotechnol, vol. 46, pp. 554-558, “Construction of L-Lysine-Overproducing Strains ofBrevibacterium Lactofermentumby Targeted Disruption of theHomandThrbGenes”, 1996.
Reinhard Kraemer, Journal of Biotechnology, vol. 45, pp. 1-21, “Genetic and Physiological Approaches for the Production of Amino Acids”, 1996.
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Bärbel Schrumpf, et al., Appl Microbiol Biotechnol, vol. 37, pp. 566-571, “Isolation and Prominent Characteristics of an L-Lysine Hyperproducing Strain ofCorynebacterium Glutamicum”, 1992.
M. Vrljic, et al., Molecular Microbiology, vol. 22, No. 5, pp. 815-826, “A New Type of Transporter with a New Type of Cellular Function: L-Lysine Export FromCorynebacterium Glutamicum”, Dec. 1, 1996.
U.S. Appl. No. 10/872,565, filed Jun. 22, 2004, Moeckel et al.
U.S. Appl. No. 10/801,847, filed Mar. 17, 2004, Hermann et al.
U.S. Appl. No. 09/903,770, filed Jul. 13, 2001, Pending.
U.S. Appl. No. 09/867,537, filed May 31, 2001, Pending.
U.S. Appl. No. 09/826,909, filed Apr. 6, 2001, Pending.

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