Nucleotide sequences for detection of serpulina hyodysenteriae

Details Nucleotide sequences for detection of serpulina hyodysenteriae Nucleotide sequences for detection of serpulina hyodysenteriae

1996-10-08

1999-02-09

Zitomer, Stephanie W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536231, 536243, 536 2431, 536 2432, 536 2433, 435 6, 435 912, C07H 2102, C07H 2104, C12Q 168, C12P 1934

Patent

active

058696300

ABSTRACT:
The invention provides a method for detecting the presence of Serpulina hyodysenteriae in a biological sample, an oligonucleotide primer and an S. hyodysenteriae-specific oligonucleotide probe useful in that method, and an article of manufacture that contains the primers and/or probe. Also provided are an about 2.3-kb DNA fragment derived from genomic DNA of S. hyodysenteriae and encoding for an about 56 kDa polypeptide, a recombinant expression vector containing the DNA fragment, the 56 kDa polypeptide and a monoclonal antibody reactive with the peptide, and a method of assaying for antibodies reactive with the 56 kDa peptide.

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