Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-04-18
2006-04-18
Myers, Carla J. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024320, C536S024330, C536S023700, C536S024310
Reexamination Certificate
active
07029853
ABSTRACT:
The invention provides purified and isolated DNA fragments fromBacillus anthracischromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods forB. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains ofBacillus, as well as accurate detection of low numbers ofB. anthracisin an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.
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Jackson, P., et al, “PCR Analysis of Tissue Samples from the 1979 Sverdlovsk Anthrax Victims: The Presence of MultipleBacillus anthracisStrains in Different Victims” Microbiology, vol. 95, pp. 1224-1229, Feb. 1998.
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Patra, G., et al., “Isolation of a Specific Chromosomic DNA Sequence ofBacillus anthracisand Its Possible Use in Diagnosis”, FEMS Immunology and Mical Microbiology 15 (1996) pp. 223-231.
Williams, JG, et al, “DNA Polymorphisms Amplified by Arbitrary Primers are useful as Genetic Markers”, (Abstract Only), Nucleic Acids Research, vol. 18, Issue 22, pp. 6531-6535.
Cheng Tu-Chen
Rastogi Vipin K.
Biffoni Ulysses John
Myers Carla J.
The United States of America as represented by the Secretary of
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