Nucleotide sequences for detection of bacillus anthracis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S023100, C536S023700, C536S024310, C536S024320

Reexamination Certificate

active

06884588

ABSTRACT:
The invention provides purified and isolated DNA fragments fromBacillus anthracischromosomal DNA, primer sets and probes derived therefrom, as well as kits and detection methods forB. anthracis. The methods of the invention provide for specific detection of anthrax over closely related strains ofBacillus, as well as accurate detection of low numbers ofB. anthracisin an environmental sample containing large amounts of non-specific DNA. The invention is applicable to food, health care, and military applications.

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patent: WO 0053804 (2000-09-01), None
The Stratagene Catalog. 1998, p. 39.*
Jackson, P., et al, “PCR Analysis of Tissue Samples from the 1979 Sverdiovsk Anthrax Victims: The Presence of MultipleBacillus AnthracisStrains in Different Victims” Microbiology, vol. 95, pp. 1224-1229, Feb. 1998.
Andersen, G., et al, “Identification of a Region of Genetic Variability AmongBacillus AnthracisStrains and Related Species” Journal of Bacteriology, Jan. 1996, vol. 178, No. 2, pp. 377-384.
Patra, G., et al., “Isolation of a Specific Chromosomic DNA Sequence ofBacillus Anthracisand Its Possible Use in Diagnosis”, FEMS Immunology and Mical Microbiology 15 (1996) pp. 223-231.
Williams, JG, et al, “DNA Polymorphisms Amplified by Arbitrary Primers are useful as Genetic Markers”, (Abstract Only), Nucleic Acids Research, vol. 18, Issue 22, pp. 6531-6535.

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