Nucleotide sequences coding for the thrE gene and process...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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Details

C435S015000, C435S069100, C435S106000, C435S320100, C435S252320, C536S023100, C536S023200

Reexamination Certificate

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06913909

ABSTRACT:
The invention relates to preferably recombinant DNA derived fromCorynebacteriumand replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out:a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes,b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, andc) Isolation of the L-threonine.

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Eikmanns B J et al.: “Molecular aspects of lysine, threonine, and isoleucine biosynthesis inCorynebacterium glutamicum”, Antonie Van Leeuwenhoek, Dordrecht, NI., Bd. 64, Nr. 2, 1993, p. 145-163.
Molenaar D et al.: “Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) fromCorynebacterium glutamicum”, European Journal of Biochemistry, Berlin, DE., Bd. 254, 1998, p. 395-403.

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