Nucleotide sequences coding for the pknB gene

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S004000, C435S106000, C435S183000, C435S193000, C435S194000, C435S252300, C435S252320, C435S320100, C536S023200

Reexamination Certificate

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07105302

ABSTRACT:
An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising:(a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2,(b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2,(c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and(d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c),and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.

REFERENCES:
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Database Swall “Online”, EBI, “M. Leprae PknB”, XP 002185694, Acc. No. P54744, Oct. 1, 1996.
Josef Cremer, et al., Applied and Environmental Microbiology, vol. 57, No. 6, pp. 1746-1752, “Control of the Lysine Biosynthesis Sequence in Corynebacterium Glutamicum as Analyzed by Overexpression of the Individual Corresponding Genes”, Jun. 1, 1991.
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Ponting, C.P. Issues in predicting protein function from sequence. Brief. Bioinform, Mar. 2001, vol. 2(1), pp. 19-29.

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