Nucleotide sequences coding for the export of branched-chain...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S069300, C435S069700, C435S071100, C435S320100, C536S023700, C536S024100

Reexamination Certificate

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06841360

ABSTRACT:
This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the groupa) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing at least one amino acid sequence of SEQ ID no. 3 or 5,b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 3 or 5,c) polynucleotide which is complementary to the polynucleotides of a), b) or c), andd) polynucleotide containing at least 15 successive bases of the polynucleotide sequences of a), b) or c).wherein the polypeptides exhibit the biological activity of the enzymes for which the brnE or bernF (sic)gene codes and a process for the fermentative production of branched-chain L-amino acids with amplification of the stated genes.

REFERENCES:
patent: 2241111 (1997-07-01), None
patent: 195 48 222 (1997-06-01), None
Database EMBL Online, acc. no. 007942, Belitsky,et al., “Branched-Chain Amino Acid Transport Protein AZLC,”J. Bacteriol. 179:5448-5457 (1997) XP 002159588.
Database EMBL Online, acc. no. 007923, Belitsky,et al., “Branched-Chain Amino Acid Transport Protein AZLD,”J. Bacteriol. 179:5448-5457 (1997) XP 002159589.
Tausch,et al., “Isoleucine Uptake in Corynebacterium ATCC 13032 Is Directed by the brnQ Gene Product,”Arch. Microbiol. 169:303-312 (1998).
Eggeling,et al., “Transport Mutants and Transport Genes ofCorynebacterium glutamicum ,” Annals of the New York Academy of Sciences 782:191-201 (1996).

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