Nucleotide sequences and methods for detection of Serpulina hyod

Details Nucleotide sequences and methods for detection of Serpulina hyod Nucleotide sequences and methods for detection of Serpulina hyod

1994-06-01

1997-12-16

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 243, 536 2431, 536 2433, C12P 1934, C12Q 168, C07H 2102, C07H 2104

Patent

active

056983948

ABSTRACT:
The invention provides a method for detecting the presence of Serpulina hyodysenteriae in a biological sample, an oligonucleotide primer and an S. hyodysenteriae-specific oligonucleotide probe useful in that method, and an article of manufacture that contains the primers and/or probe. Also provided are an about 2.3-kb DNA fragment derived from genomic DNA of S. hyodysenteriae and encoding for an about 56 kDa polypeptide, a recombinant expression vector containing the DNA fragment, the 56 kDa polypeptide and a monoclonal antibody reactive with the peptide, and a method of assaying for antibodies reactive with the 56 kDa peptide.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4748019 (1988-05-01), Lysons
patent: 4764370 (1988-08-01), Fields et al.
patent: 4818680 (1989-04-01), Collins et al.
patent: 4851331 (1989-07-01), Vary et al.
patent: 4999191 (1991-03-01), Glisson et al.
patent: 5034315 (1991-07-01), Jensen et al.
patent: 5298392 (1994-03-01), Atlas et al.
patent: 5306616 (1994-04-01), Lupski et al.
Achacha et al., J. Vet. Diag. Invest., 3:211-214 (1991) is cited in the specification at p. 3, line 27-28.
Baum and Joens, Infect. Immun., 25:792-796 (1979) is cited in the specification at p. 2, line 3.
Belanger et al., J. Clin. Microbiol., 29:1727-1729 (1991) is cited in the specification at p. 3, lines 28-29 and p. 4, line 1.
Boyden et al., "Cloning and Characterization of Treponema hyodysenteriae Antigens and Protection in a CF-1 Mouse Model by Immunization with a Cloned Endoflagellar and Antigen", Infect. Immun., 57:3808-3815 (1989) is cited in the specification at p. 5, lines 11-12.
Burrows and Lemcke, Vet. Rec., 108:187-189 (1981) is cited in the specification at p. 4, line 2 and lines 14-15.
Caputa et al., J. Clin. Microbiol., 29:2418-2413 (1991) is cited in the specification at page 47, line 25-26.
Chenngappa et al., "Laboratory Procedures for the Diagnosis of Swine Dysentary", Report of the Committee on Swine Dysentery, American Association of Veterinary Laboratory Diagnosticians, Committee on Swine Dysentery, Aug. (1989) is cited in the specification at p. 2, lines 10-13.
Chien et al., J. Bacteriol. 127:1550 (1976) is cited in the specification at p. 27, lines 20-21.
Combs and Hampson, Res. Vet. Sci., 50:286-289 (1991) is cited in the specification at p. 5, line 26.
Curtiss III et al., Infection and Immunity, 55:3035 (1987) is cited in the specification at p. 37, line 18.
Cwyk et al., Arch. Microbiol., 122:231-239 (1979) is cited in the specification at p. 45, line 27.
Dougan et al., J. Infec. Dis., 158:1329 (1988) is cited in the specification p. 37, line 19.
Duhamel et al., J. Vet. Diagn. Invest., 4:285-292 (1992) is cited in the specification at p. 58, line 35 and p. 63, line 33.
Edwards et al., J. Bacteriol., 170:3991 (1988) is cited in the specification at p. 37, lines 19-20.
Elder et al., J. Clin. Micro., 32:1497 (1994) is cited in the specification at p. 16, line 29; p. 22, line 18-19; p. 27, line 33; and p. 63, line 6 and line 34.
Fisher and Olander, Am. J. Vet. Res., 46:450-455 (1981) is cited in the specification at p. 2, line 27.
Flores et al., "Identification of Human Rotavirus Serotype by Hybridization to Polymerase Chain Reaction-Generated Probes Derived from a Hyperdivergent region of the Gene Encoding Outer Capsid Protein VP7", J. Virol., 64:4021-4024 (1990) is cited in the specification at p. 20, lines 11-12.
Harris and Lysons, Diseases of Swine, 7th ed., Iowa State University Press (Ames, IA), at pp. 599-616 (1992) is cited in the specification at p. 1, line 34-35.
Kinyon and Harris, Int. J. Syst. Bact., 29:102-109 (1979) is cited in the specification at p. 2, line 18; p. 44, line 23; and p. 45, lines 2-3.
Hopp et al., Mol. Immunol., 20:483 (1983) is cited in the specification at p. 62, line 8.
Hugo et al., J. Clin. Microbiol., 25:26-30 (1987) is cited in the specification at p. 61, lines 1-2, lines 22-23, and lines 30-31.
Hunter et al., Vet. Rec., 104:383-384 (1979) is cited in the specification at p. 3, lines 29-30.
Islam et al., "Detection of Shigella dysenteriae Type 1 and Shigella flexneri in Feces by Immunomagnetic Isolation and Polymerase Chain Reaction", J. Clin. Micro., 30:2801-2806 (1992) is cited in the specification at p. 27, lines 11-12 and p. 28, lines 20-21.
Jensen et al., "Detection and Identification of Treponema hyodysenteriae by Using Oligodeoxynucleotide Probes Complementary to 16S rRNA", J. Clin. Microbiol., 28:2717-2721 (1990) is cited in the specification at p. 5, lines 17-18.
Jones et al., J. Clin. Microbiol., 24:1071-1074 (1986) is cited in the specification at p. 45, lines 19-20.
Judd et al., Appl. Env. Microbiol., 59:1702 (1993) is cited in the specification at p. 28, lines 25-26.
Katz et al., "Colorimetric Diagnosis of Prolonged Bluetongue Viremia in Sheep, Using an Enzyme-linked Oligonucleotide Sorbent Assay of Amplified Viral Nucleic Acids", Am. J. Vet. Res., 54:2021-2026 (1993) is cited in the specification at p. 28, line 23 and p. 32, line 4.
Kinyon and Harris, Infect. Immun., 15:638-646 (1977) is cited in the specification at p. 4, line 3 and p. 44, lines 24-25.
Kohler and Milstein, Nature 256:496-97 (1975) is cited in the specification at p. 39, line 3.
Koopman et al., "Cloning and DNA Sequence Analysis of a Serpulina (Treponema) hyodysenteriae Gene Encoding a Periplasic Flagellar Sheath Protein", Infect. Imm., 60:2920-2925 (1992) is cited in the specification at p. 51, line 9-10.
Kunkle et al., J. Clin. Microbiol., 24:669-671 (1986) is cited in the specification at p. 46, line 25 and p. 47, lines 22-23.
Kunkle et al., J. Clin. Microbiol., 24:669-671 (1986) is cited in the specification at p. 3, line 13 and p. 59, line 31.
Kunkle et al., J. Clin. Microbiol., 26:2357-2360 (1988) is cited in the specification at p. 3, line 13 and p. 59, line 31.
Lee et al., Vet. Microbiol., 34:35-46 (1993) is cited in the specification at p. 45, line 13.
Lemcke and Burrows, Vet. Rec., 104:548-551 (1979) is cited in the specification at p. 4, lines 13-14.
Li et al , J. Clin. Microbiol., 29:2794-2797 (1991) is cited in the specificition at p. 2, line 5 and p. 44, line 35.
Lory, J. Bacteriol., 174:3423-3428 (1992) is cited in the specification at p. 50, lines 31-32.
Luneberg et al., "Detection of Mycoplasma pneumoniae by Polymerase Chain Reaction and Nonradioactive Hybridization in Microtiter Plates" J. Clin. Micro. 31: 1088-1094 (1993) is cited in the specification at p. 28, line 22.
"Reaction and Nonradioactive Hybridization in Microtiter Plates", J. Clin. Micro. 31:1088-1094 (1993) is cited in the specification at p. 28, line 22.
Lymbery et al., Vet. Microbiol., 22:89-99 (1990 is cited in the specification at p. 4, line 4.
Lysons, Vet. Rec., 129:314-315 (1991) is cited in the specification at p. 4, lines 19 and 28.
Mapother and Joens, J. Clin. Micro., 22:161-164 (1985) as cited in the specification at p. 2, line 4.
Mowat et al., Immunology Today, 12:383 (1991) is cited in the specification at p. 35, line 37.
Muir et al., "Cloning and Expression of a Serpula (Treponema) hypayseneriae Hemolysin Gene", Infect. Immun., 60:529-535 (1992) is cited in the specification at p. 51, lines 5-6.
Mullis and Faloona, Methods Enzymol. 155:335 (1987) is cited in the specification at p. 27, line 14.
Owen, Iowa State J. Res., 62:293-311 (1987) is cited in the specification at p. 1, lines 31-32.
Picard et al., Can. J. Microbiol., 26:985-991 (1980) is cited in the specification at p. 4, lines 4-5.
Ramanathan et al., "Identification and Partial Characterization of a Group of Weakly .beta.-hemolytic Intestinal Spirochetes of Swine Distinct from Serpulina innocens Isolate", Vet. Microbiol., 37:53-64 (1993) is cited in the specification at p. 4, lines 5-6, p. 45, line 14; p. 47, lines 4-5; and page 54, line 17.
Rychlik and Rhoades, Nucleic Acid Res., 17:8543-8551 (1989) is cited in the specification at p. 55, lines 24-27.
Saiki et al., Science 239:487 (1988) is cited in the specification at p. 27, lines 19-20.
Sellwood et al., "Outer Envelope and Axial Filiment Polypeptides of T. hydrodysenteriae, Characterization and Molecular Cloning", Proc. Inte

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