Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-07-01
1999-12-07
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 6, 435 911, 435 912, 435 915, 536 231, 536 243, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104
Patent
active
059981385
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a specific nucleic sequence of Campylobacter coli, as well as to applications of this sequence as specific nucleotide probe for the detection of sequences of Campylobacter coli or of fragments of this sequence as nucleotide primers for the amplification of DNA or RNA of Campylobacter coli in a biological specimen.
2. Description of the Background Art
Campylobacter infections are very common all over the world, affecting man as well as wild or domestic animals.
Although discovered at the start of the twentieth century, the bacteria now called Campylobacter were unrecognized for a long time, because their characteristics rendered their identification and their culture difficult. First isolated in sheep and cattle and called Vibrio fetus and then, later, Campylobacter fetus, it was not until the start of 1946 that the first cases of human campylobacterioses were described, but it was not until the start of 1972, when selective media for Campylobacter had begun to be perfected, that the importance of Campylobacter infections had been able to be proved and recognized.
Since the naming of the species type Campylobacter fetus, about a dozen other species and subspecies have been discovered, the exact number varying according to the authors and the taxonomic methods, which often propose novel classification criteria. Among these species, the most frequently encountered in human and/or animal pathology are Campylobacter jejuni, Campylobacter coli and Campylobacter fetus.
At present, Campylobacter jejuni and Campylobacter coli are frequently responsible for infectious diarrhoea in man.
The "national surveillance system of Campylobacter infections", set up in France in 1986, publishes each year an assessment making evident the principal epidemiological and clinical data of the cases reported.
In the human species, the major symptom of intestinal infection with C. jejuni or with C. coli is diarrhoea which, in the most serious cases, can involve serious losses of water, which can be particularly dangerous for children and infants, who are very sensitive to dehydration. However, Campylobacter enteritis is often without complications and diarrhoeas can even cease spontaneously at the end of one week. However, the coprocultures can remain positive up to several weeks or even months, and, in 5 to 10% of the cases, relapses can occur. Treatment and rigorous follow-up are then essential, especially for subjects who are immunodepressed or have serious disorders (AIDS, cirrhosis of the liver, cancer, leukaemia, etc.), in which Campylobacter can behave like opportunistic bacteria.
Other consequences of C. jejune or C. coli infections have also been described, although rarer or more exceptional: mesenteric adenitis, cholecystitis, urinary infections, meningites, septicaemias, erythema nodosum or Guillain-Barre syndrome, etc.
In animals, the Campylobacter usually live in commensals in the alimentary canal of numerous species: cattle, sheep, pigs, poultry, wild birds, dogs and cats. These animals, whether ill or healthy carriers, form a large reservoir of germs, and thus an important risk of contamination. In the case of obvious infections, in cattle and sheep, C. jejuni is known, since the first description in 1931, as being the cause of "bovine dysentery", which can have the result, apart from the effect on the cattle, of transmission to man through the dissemination of germs in the environment of the animals (ground, water). Even for the asymptomatic animals, "healthy carriers", transmission to man can take place: either by direct contact with these animals or their excrement, or by consumption of contaminated food or water (meats contaminated during their preparation and badly cooked, non-pasteurized milk, polluted water, etc.).
From a prevention viewpoint, it is important then, both in man and in animals, to be able to identify the pathogen C. jejuni or C. coli as soon as possible, in order to prevent, by means of adequate measures, any co
REFERENCES:
patent: 5654416 (1997-08-01), Cummins
Chevrier et al, "Identification and Classification of Campylobacter Strains by Using Nonradioactive DNA Probes," Journal of Clinical Microbiology, pp. 321-326, (Feb. 1989).
Eyers et al, "Discrimination Among Thermophilic Campylobacter Species by Polymerase Chain Reaction Amplification", Journal of Clinical Microbiology, pp. 3340-3343 (Dec. 1993).
Gourse, R.L. et al. Site-directed mutagenesis of ribosomal RNA. J. Mol. Biol. 159:397-416, 1982.
Guesdon Jean-Luc
Stonnet Veronique
Institut Pasteur
Johannsen Diana
Myers Carla J.
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