Nucleotide sequence for creatinine deiminase and method of use

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S252300, C435S252310, C435S252330, C435S254110, C435S320100, C435S325000, C435S348000, C435S410000, C536S023200

Reexamination Certificate

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10689564

ABSTRACT:
The present invention relates to the discovery of novel genes encoding a polypeptide having creatinine deiminase activity and methods of use. Also disclosed is a kit using the novel sequences for determining the concentration of creatinine in a sample.

REFERENCES:
patent: 1325958 (2003-07-01), None
patent: 07143881 (1995-06-01), None
Gottschalk et al., (1991) Creatinine deiminase (EC 3.5.4.21) from bacterium BN11: purification, properties and applicability in a serum/urine creatinine assay.Clin Chim Acta. 204(1-3): 223-38.
Farrow et al., (1995) Phylogenetic evidence that the gram-negative nonsporulating bacteriumTissierella(Bacteroides) praeacuta is a member of theClostridium subphylumof the gram-positive bacteria and description ofTissierella creatininisp. nov.Int J Syst Bacteriol. 45(3): 436-40.
Tanganelli et al., (1982) Enzymic assay of creatinine in serum and urine with creatinine iminohydrolase and glutamate dehydrogenase.Clin Chem. 28(7): 1461-4.
Austin and Huber, Genbank Database; accession S56903, publicly available Jun. 28, 1993.

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