Nucleotide sequence encoding a 52 kDa Ro/SSA autoantigen

Details Nucleotide sequence encoding a 52 kDa Ro/SSA autoantigen Nucleotide sequence encoding a 52 kDa Ro/SSA autoantigen

1992-09-16

2000-08-29

Horlick, Kenneth R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 241, 536 2431, 435 6, 435270, 4352523, 4353201, 530358, 5303892, C07H 2104, C12Q 168, C12N 108, C08G 6382

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active

061110884

ABSTRACT:
Complementary DNA encoding a 52 kDa form of a protein present in the human Ro/SSA ribonucleoprotein complex has been cloned. A lambda gt11 cDNA library made from human thymocyte mRNA was screened with serum from a SLE patient and two immunoreactive clones were isolated. These clones reacted with other patient sera which had anti-52 kDa Ro/SSA antibodies and with affinity purified anti-52 kDa Ro/SSA antibodies. Moreover, affinity purified antibodies eluted from fusion proteins of the isolated clones reacted only with the 52 kDa protein of lymphocytes in the Western blot. Ro/SSA RNAs were also precipitated with these affinity purified antibodies, further confirming that the clones encode a 52 kDa Ro/SSA antigen. The sequence differs from the previously reported 60 kDa Ro/SSA gene.

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