Nucleotide sequence coding for an outer membrane protein from Ne

Details Nucleotide sequence coding for an outer membrane protein from Ne Nucleotide sequence coding for an outer membrane protein from Ne

1991-09-05

1994-02-15

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

424 92, 536 237, 435 691, 4353201, 530350, A61K 3902, A61K 3900, C07H 1512, C12P 2106

Patent

active

052864847

ABSTRACT:
The present invention is concerned with a method for the isolation of a nucleotide sequence which codes for a protein having a molecular weight of about 64,000 daltons, which is located on the outer membrane of N. meningitidis, as well as with the recombinant DNA obtained therefrom, which is used for the transformation of a host microorganism. The technical object pursued with the invention is the identification of a nucleotide sequence coding for a highly conserved and common protein for the majority of pathogenic Neisseria strains, the production of this protein with a high level of purity and in commercially useful amounts using the recombinant way, so that it can be used in diagnostic methods and vaccine preparations with a broad immunoprotection spectrum.

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