Nucleotide sequence capable of inducing high levels of translati

Details Nucleotide sequence capable of inducing high levels of translati Nucleotide sequence capable of inducing high levels of translati

1987-04-21

1991-01-22

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 43525231, 4353201, 435231, 536 27, 935 29, 935 44, 935 45, 935 46, 935 73, 935 74, C12P 2100, C14N 1500, C07H 1512

Patent

active

049870696

ABSTRACT:
Nucleotide sequences capable of inducing high levels of translation of a heterologous gene in Bacillus subtilis and Escherichia coli and a method of preparing heterologous proteins comprising cultivation in a suitable culture medium of cells of Bacillus subtilis and Escherichia coli transformed by hybrid plasmids in which the nucleotide sequence is situated between the ribosome recognition site and the starting triplet (ATG) of the coding structural gene for the desired proteins.

REFERENCES:
patent: 4529695 (1985-07-01), Weisblum
patent: 4626510 (1986-12-01), Grandi
Kiss, A. et al, Nuc. Acids Res., vol. 13, pp. 6403-6421, 1985.
Stahl, M. et al, J. Bacteriology, vol. 158, pp. 411-418, 1984.
Breunig, K. et al, Gene 20, pp. 1-10, 1982.
Roberts, T. et al, Proc. Natl. Acad. Sci., vol. 76, pp. 760-764, 1979.
Grandi, G. et al, Plasmid, vol. 16, pp. 1-14, Jul. 1986.

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