Nucleotide mixture for improved nucleic acid amplification...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S091100, C435S091200

Reexamination Certificate

active

07364854

ABSTRACT:
The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates.

REFERENCES:
patent: 5399491 (1995-03-01), Kacian et al.
patent: 5405950 (1995-04-01), Mock et al.
patent: 5411876 (1995-05-01), Bloch et al.
patent: 5633364 (1997-05-01), Mock et al.
patent: 6271004 (2001-08-01), Warthoe
patent: 6312928 (2001-11-01), Van Gemen et al.
patent: 2001/0041334 (2001-11-01), Rashtchian et al.
patent: 2002/0119534 (2002-08-01), Ihlenfeldt et al.
patent: 19612779 (1997-10-01), None
patent: 1026261 (2000-08-01), None
patent: WO98/44161 (1998-10-01), None
patent: WO03/054209 (2003-07-01), None
Green (“General PCR Protocol” http://www.biology.Isa.umich.edu/research/labs/maddock/protocols/PCR/general—pcr—protocol.html. Feb. 17, 1996).
International Search Report, European Patent Office, in International Application No. PCT/US2005/002414 (form PCT/ISA/210) (date of mailing: Feb. 6, 2006).
Eds. Ausubel et al., “A Compendium of Methods from Current Protocols in Molecular Biology” Short Protocols in Molecular Biology, 2ndedition., Chapter 15, pp. 15.1-15.27, John Wiley & Sons, New York (1991).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Nucleotide mixture for improved nucleic acid amplification... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleotide mixture for improved nucleic acid amplification..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleotide mixture for improved nucleic acid amplification... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2745756

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.