Nucleotide hybridization assay for protozoan parasites

Details Nucleotide hybridization assay for protozoan parasites Nucleotide hybridization assay for protozoan parasites

1987-04-16

1989-01-31

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 435 91, 435258, 435947, 43525233, 435320, 435235, 436504, 436505, 436501, 935 19, 935 20, 935 22, 935 27, 935 31, 935 73, 935 77, 935 78, C12Q 168, C12N 120, C12N 110

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048015300

ABSTRACT:
The present invention provides a method for detection of protozoan parasites in blood or other specimen from their mammalian hosts. The method comprises nucleic acid hybridization of repetitive nuclear DNA fragments of the parasites. Hybridization probes have been prepared for this purpose by cloning the repetitive nuclear elements that are species-specific in appropriate vectors. The sensitivity of these probes has been increased by further sub-cloning to make them capable of cascade hybridization. The assay is highly specific and sensitive for detection of disease-causing protozoan parasites of the commonly occurring Trypanosomatidae of the genus Leishmania and genus Trypanosoma, as well as for malaria-causing protozoan and other parasitic microorganims of mammals.

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