Nucleotide based endonucleases

Details Nucleotide based endonucleases Nucleotide based endonucleases

1994-03-28

1999-10-26

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435 6, 435 9131, 4353201, 435375, 536 231, 536 232, 536 245, C12N 1585, C12Q 168, C07H 2104

Patent

active

059727012

ABSTRACT:
This invention provides catalytic molecules capable of cleaving target nucleotide sequences. More specifically, the invention provides an endonuclease having nucleotide sequences which are of sufficient length to allow hybridization to a target nucleotide sequence desired to be cleaved. The endonuclease contains a catalytic region comprising ribonucleotides and/or deoxyribonucleotides, or derivatives thereof which act to cleave a phosphodiester bond of the substrate nucleotide sequence. The catalytic region comprises nucleotides or derivatives thereof which are linked by linking groups which may comprise ribonucleotides, deoxyribonucleotides or combinations thereof.
The endonucleases of the invention are useful in the cleavage of target RNAs associated with disease in humans and animals and in the inactivation of RNA transcripts in eukaryotic and prokaryotic cells, as well as the cleavage of RNA transcripts in-vitro.

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