Nucleoside oxidase and process for making same, and process and

Details Nucleoside oxidase and process for making same, and process and Nucleoside oxidase and process for making same, and process and

1981-08-12

1983-05-24

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 15, 435 18, 435 25, 435 21, 435 28, 435191, 435810, 435877, 435136, 435128, C12N 906, C12P 740, C12P 1300, C12Q 126, C12Q 128, C12Q 134, C12Q 142, C12Q 168, C12R 140

Patent

active

043851126

ABSTRACT:
A microorganism strain B-0781 belonging to the genus Pseudomonas isolated from a soil sample from an onion field in Japan, produces a novel enzyme nucleoside oxidase having substrate specificity on various nucleosides and enzyme action to catalyze enzymatic reactions involving various nucleosides. The novel nucleoside oxidase is produced by culturing a nucleoside-oxidase-producing microorganism belonging to the genus Pseudomonas in a nutrient medium containing assimilable carbon and nitrogen and inorganic salt, and isolating the thus-formed nucleoside oxidase from the cultured cells. Various nucleoside-5'-carboxylic acids can be produced, by incubating the novel nucleoside oxidase with a nucleoside having a 5'-hydroxymethyl group, in an aqueous medium under aerobic conditions, and isolating the thus-formed nucleoside-5'-carboxylic acid from the incubation medium. Assay methods for nucleosides in liquid samples are provided, which comprise incubating the sample with nucleoside oxidase, thereby consuming oxygen and generating hydrogen peroxide and nucleoside-5'-carboxylic acid by acting on nucleoside, and quantitatively determining consumed oxygen or liberated hydrogen peroxide. A kit for nucleoside assay is provided, which contains the recited ingredients.

REFERENCES:
Liu et al., Biochemistry, 11(11), 2172-2176 (1972).

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