Nucleophilic polysubstituted aryl acridinium ester conjugates us

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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435 71, 435 74, 435 6, 436500, 436505, 436518, 436544, 436546, 436800, 436817, 546107, G01N 33566

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055389015

ABSTRACT:
This invention is directed to the novel assay methods utilizing nucleophilic polysubstituted aryl acridinium ester conjugates as the tracers. Conjugates prepared by covalent coupling of novel nucleophilic polysubstituted aryl acridinium esters with biological compounds including small organic molecules such as Vitamin B12, folate, cortisol, estradiol, and thromboxane B2, were found useful in the development of highly sensitive assays for the analytes of diagnostic interest.

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