Nucleolipids and use thereof, and devices for nucleic acid...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Details

C435S005000, C435S007100, C435S007200, C536S023100, C536S024300, C536S025300, C536S026600, C422S067000

Reexamination Certificate

active

07914991

ABSTRACT:
The invention relates to a method for isolating and/or identifying known or unknown, nucleic acid sequences (target sequences) which are marked, optionally, with reporter groups, by base specific hybridisation with, essentially, complementary sequences, which belong to a library or sequences. The sample sequences are characterised in that they support, on one of the termini thereof (5′-ends or 3′-ends, preferably on 5′-ends), a single double or multi-chained lipid part, which spreads in a monomolecular layer on a liquid gas or liquid-liquid phase boundary layer. The invention also relates to a system for detecting or isolating nucleic acids.

REFERENCES:
patent: 5976567 (1999-11-01), Wheeler et al.
Miao et al., Molecular Recognition of Nucleoliplid Monolayers of 1-(2- Octadecyloxycarbonylethyl) cytosine to Guanosine at the Air-water Interface and Langmuir-Blodgett Films, Bd. 19, 17, May 2003, Sieten 5389-5396.
Huang et al. Molecular Recognition of Nucleolipid Amphiphile Ocadecanoyl Ester of 1-(2-carboxyethyl) adenine to the complemenetary nucleobases. Part IBd. 326, Nr. 1-2, Aug. 4, 1998, Seiten 217-222.
Rosemeyer et al. A Nucleolipid with antiviral Acycloguanosine as head Group Synthesis and Liposome Formation, Bd. 24, Nr. 6, 1985, Seiten 501-502.
Davis et al., Structured thin Films as Functional Components within Biosensors, Bd. 21, No. 1, Nov. 18, 2004, Seiten 1-20.

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