Nucleic acids sequence, stress-induced proteins and uses...

Details Nucleic acids sequence, stress-induced proteins and uses... Nucleic acids sequence, stress-induced proteins and uses...

1998-02-03

2001-12-25

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Vector, per se

C536S023100

Reexamination Certificate

active

06333191

ABSTRACT:

Publications and manuscripts which are attached and co-filed herewith are also incorporated by reference in their entirety.
The parent application discloses cold-shock proteins which are induced by cold-shock following a shift to lower temperature from physiological growth temperatures.
E. coli
was shown to grow at low temperatures after cold-shock induction. Other features of these proteins and their promoters are disclosed in the parent application.
SUMMARY OF THE INVENTION
The present invention further describes these and other members of a family of stress-induced, in particular cold-shock induced proteins (Csp), their synthesis following a shift from physiological growth temperature of a microorganism to temperatures below physiological, nucleotide sequences of stress-induced and cold-shock genes, deduced amino acid sequences thereof and cold-shock induced and other promoters. The invention also describes the expression of proteins other than the cold-shock proteins under the direction of cold-shock promoters. Various utilities of the cold-shock proteins and promoters are described.


REFERENCES:
Goldstein et al. P.N.A.S. 87:283-287, Jan. 1990.*
Yee et al. Molecular Microbiology 11(5) 833-839, Mar. 1994.*
Maniak et al. Jan., 1988; Mol. Cell. Biol. 8(1):153-159.*
Remaut et al. 1981; Gene 15:81-93.

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