Nucleic acids encoding transcription factor APRF (acute phase re

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525233, 4353201, 435356, 435358, 435365, 536 235, C12P 2106, C12N 1563, C12N 500, C07H 2104

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057190429

ABSTRACT:
Mammal transcription factor APRF, process for the preparation of it, DNAs encoding the product, replication and expression vector comprising the DNA, host cells transformed or tranfected with the replication and expression vector, evaluating and screening method for searching an inhibitory agent on the function of APRF and an inhibitory agent against the function of the APRF. The peptide of the present invention (APRF) may be useful for complement or suppression of the function of APRF, screening an inhibitory agent against the function of APRF. Inhibiting agent containing the product as active ingredient of the present invention may be also useful for treatment of diseases related to cytokine such as IL-6, i.e., inflammatory diseases.

REFERENCES:
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IL-6DBP, a Nuclear Protein . . . , Valeria Poli et al, Cell, vol. 63, pp. 643-653, Nov. 1990.
A member of the C/EBP family . . . , Shigemi Kinoshita et al, Proc. Natl. Acad. vol. 89 pp. 1473-1476, Feb. 1992.
Acute-Phase Response Factor, Ursula M. Wegenka et al, Molecular 7 Cellular Bio., pp. 276-288, Jan. 1993.
Molecular Cloning of APRF . . . , S. Akira et al, Cell, vol. 77, pp. 63-71, Apr. 1994.
Maniatis et al. Hybridization to nitrocellulose filters containing replicas of bacteriophage plaques or bacterial colonies in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 326-328, 1982.
Zhong et al. Stat3: A STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and Interleukin-6. Science 264:95-98, 1994.
Wegenka et al. The Interleukin-6-activated acute-phase-response-factor is antigenically and functionally related to members of the signal transducer and activator of transcription (STAT) family. Molecular and Cellular Biology. 14(5):3186-3196, 1994.
Zhong et al. Stat3 and Stat4: Members of the family of signal transducers and activators of transcription. PNAS USA. 91(11):4806-4810, 1994.

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