Nucleic acids encoding tospovirus genome and expression thereof

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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435 691, 4351723, 4353201, 435418, 435419, 536 2372, A01H 500, C12N 1582, C12N 1533

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059396008

ABSTRACT:
Recombinant DNA constructs comprising a DNA coding for transcription into an RNA sequence of tospoviruses or into RNA sequences related thereto, the use of such DNA constructs to transform plants having reduced susceptibility to tospovirus infection and probes for the isolation of tospovirus or diagnosis of pant tospovirus diseases.

REFERENCES:
German et al. 1988 "Dot Blot Detection of Tomato Spotted Wilt Virus Uing Cloned Complementary DNA Probes" Phytopathology 78:1599.
Ronco et al. 1989 "Cloned complementary DNA probes for the detection of Tomato Spotted Wilt Virus" Phytopathology 79(11):1309-1313.
DeHaan et al. 1989 "Molecular Cloning and Terminal Sequence Determination of the S and M RNAs . . . " J. Gen. Virol. 70:3469-3473.
DeHaan et al. 1990 "The S RNA Segment of Tomato Spotted Wilt Virus Has An Ambisense Character" J. Gen. Virol. 71:1001-1007.
Huguenot et al. 1990 "Detection of Tomato Spotted Wilt Virus using monclonal antibodies and riboprobes" Arch. Virol. 110(1/2):47-62.
Wilson 1993 "Strategies to protect plants against viruses" Proc. Natl. Acad. Sci USA 90:3134-3141.
Nejidat et al. 1990 "Engineered resistance against plant virus disease" Physiol. Plantarum 80:662-668.
Lewin 1987 "When does homology mean something else" Science 273:70.
Cuozzo et al. 1988 "Viral Protection in transgenic tobacco plants expressing the cucumber mosaic virus coat . . . " Bio/Technology 6:549-557.
Verkleig et al. 1982 "Evidence that Tomato Spotted Wilt Virus RNA is a positive strand" J. Gen. Virol. 48:329-338.
DeHaan et al., 1989, "Molecular Cloning and Terminal Sequence Determination of the S and M RNAs of Tomato Spotted Wilt Virus", J. Gen. Virol. 70:3469-3473.
German et al., 1988 "Dot Blot Detection of Tomato Spotted Wilt Virus Using Cloned Complementary DNA Probes" Phyto path. 78:1599.
Verkleij et al., 1983, J. Gen. Virol., 64:677-686.
Nejidat et al., 1990, "Engineered Resistance Against Plant Virus Deseases" Physiol. Plant. 80:662-668.
Cuozzo et al., 1988 "Viral Protection In Transgenic Tobacco Plants Expressing The Cucumber Mosaic Virus Coat Protein or it's Antisense DNA" Bio/Technology 6:549-557.
Wilson, M.T. 1993, "Strategies to Protect Crop Plants against Viruses", Proc. Natl. Acad. Sci. 90:3134-3141.
Hugenot et al., 1990, "Detection of Tomato Spotted Wilt Virus using Monoclonal Antibodies and Riboprobes", Arch. Virol., 110:47-62.
Ronco et al., 1989, "Cloned cDNA Probes for The Detection of Tomato Spotted Wilt Virus" Phytopath.: 79:1309-1313.

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