Nucleic acids encoding sorting nexins and methods of using same

Details Nucleic acids encoding sorting nexins and methods of using same Nucleic acids encoding sorting nexins and methods of using same

1996-04-01

1998-09-08

Carlson, Karen Cochrane

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435325, 4353201, 536 231, 530300, 530350, C12P 2156, C12N 1500, C07H 1700, C07K 1400

Patent

active

058044124

ABSTRACT:
The present invention provides substantially purified members of the sorting nexin (SNX) family of proteins, which are involved in down regulating the expression of cell surface receptors and directing translocation of the receptor to a lysosome in the cell, as well as active fragments of an SNX and nucleic acid molecules encoding an SNX or an active fragment thereof. In addition, the invention provides nucleotide sequences that hybridize under relatively stringent conditions to a nucleic acid molecule encoding one or more members of the SNX family of proteins. The invention further provides vectors containing a nucleic acid molecule encoding a member of the SNX family of proteins or an active fragment thereof and provides host cells containing such vectors. The present invention further provides a method of modulating the expression of a cell surface receptor by expressing a nucleic acid molecule of the invention in a cell that can express the receptor. In addition, the invention provides a method of identifying an endosomal fraction of a cell by contacting the cell or a fraction of the cell with a probe, under suitable conditions, which allow binding of the probe to an endosomal fraction, if present, such binding being detectable.

REFERENCES:
Gray et al. 1983 Nature 303: 722-725.
Lee et al Aug. 1995 PMAS 92: 8303-8307.
Hoffman et al. 1994 Mol. Cell Biol 14(6): 3695-3706.
Letourneur, F. and Klausner, R., "A Novel Di-Leucine Motif and a Tyrosine-Based Motif Independently Mediate Lysosomal Targeting and Endocytosis of CD3 Chains." Cell 69:1143-1157 (1992).
Johnson, K. and Kornfeld, S. "The Cytoplasmic Tail of the Mannose 6-Phosphate/Insulin-like Growth Factor-II Receptor has Two Signals for Lysosomal Enzyme Sorting in the Golgi." J. Cell Biol. 119:249-257.
Ekena, K. and Stevens, T. "The Saccharomyces Cerevisiae MVP1 Gene Interacts with VPS1 and is Required for Vacuolar Protein Sorting." Mol. Cell. Biol. 15:1671-1678 (1995).

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