Nucleic acids encoding sialytranferases from C. jejuni

Details Nucleic acids encoding sialytranferases from C. jejuni Nucleic acids encoding sialytranferases from C. jejuni

2007-01-30

2007-01-30

Swope, Sheridan (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S193000

Reexamination Certificate

active

10734719

ABSTRACT:
This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example,Campylobacterspecies, includingC. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.

REFERENCES:
patent: 5352670 (1994-10-01), Venot et al.
patent: 5374541 (1994-12-01), Wong et al.
patent: 5545553 (1996-08-01), Gotschlich
patent: WO 92/16640 (1992-10-01), None
patent: WO 96/32491 (1996-10-01), None
patent: WO 99/49051 (1999-09-01), None
patent: WO 00/46379 (2000-08-01), None
Gilbert et al, Biosynthesis of ganglioside mimics inCampylobacter jejuniOH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-mhz (1)h and (13)c NMR analysis. J Biol Chem. Feb. 11, 2000;275(6):3896-906.
Goodyear et al, Monoclonal antibodies raised against Guillain-Barre syndrome-associatedCampylobacter jejunilipopolysaccharides react neuronal gangliosides and paralyze muscle-nerve preparations. J Clin Invest. Sep. 1999;104(6):697-708. Erratum in: J Clin Invest Dec. 1999;104(12):1771.
Yeh et al, Absorbance- and light-based solid-phase assays for CMPNeuAc:Galβ 1-4GlcNAc-R α -2,3-sialytranferase. Anal Biochem. Apr. 5, 1996;236(1):126-33.
Guo et al, Protein tolerance to random amino acid change. Proc Natl Acad Sci U S A. Jun. 22, 2004;101(25):9205-9210. Epub Jun. 14, 2004.
Aspinall et al.; “Chemical Structures of the Core Regions ofCampylobacter jajuniSerotypes 0:1, 0:4, 0:23, and 0:36 Lipopolysaccharides”;European Journal of Biochemistry; May, 1993; pp. 1017-1027; vol. 213, No. 3.
Aspinall et al.; “Lipopolysaccharides fromCampylobacter jejuniAssociated with Guillain-Barre Syndrome Patients Mimic Human Gangliosides in Structure”;Infect. Immun.; 1994a; pp. 2122-2125; vol. 62.
Aspinall et al.; “Lipoplysachharides ofCamplylobacter jejuniSerotype O: 19: Structures of Core Oligosaccharide Regions from the Serostrain and Two Bacterial Isolates from Patients with the Guillain-Barre Syndrome”;Biochemistry; 1994b; pp. 241-249; vol. 33.
Aspinall et al.; “Lipopolysaccharides ofCampylobacter jejuniSerotype O:19: Antigen Chains from the Serostrain and Two Bacterial Isolates from Patients with the Gullain-Barre Syndrome”;Biochemistry; 1994c; pp. 250-255; vol. 33.
Belunis et al.; “Biosynthesis of Endotoxin”;J. Biol. Chem.: 1992; pp. 9988-9997; vol. 287.
Gaudino et al.; “A Novel and Efficient Synthesis of Neolacto Series Gangliosides 3 '-nLM1and 6'-nLM1”;J. Am. Chem.Soc.: 1994: pp. 1149-1150; vol. 116.
Gilbert et al., “Biosynthesis of Ganglioside Mimics inCampylobacter jejuniOH4384”;The Journal of Biolofical Chemistry: Feb. 11, 2000; pp. 2896-3906; vol. 275, No. 6: The American Society for Biochemistry and Molecular Biology, Inc.; USA.
Gilbert et al.; “Cloning of the Lipooligosaccharide α-2, 3-Slatytransferase from the Bacterial PathogensNeisseria meningitidisandNelsserla gonorrhoeae”;J. Biol. Chem.: 1996; pp. 28271-28276; vol. 271.
Gilbert et al.; EMBL Acc# AY044868 2002. Alignment with SEQ ID No: 3.
Ito et al.; “Synthesis of Bioactive Sialosides”;Pure Appl. Chem.: 1993, p. 753; vol. 65.
Kuroki; “Camplylobacter jejuniStrains from Patients with Gullain-Barre Syndrome Belong Mostly to Penner Serogroup 19 Contain β-N-Acetylglucosamine Residues”;Ann. Neurol.1993; pp. 243-247; vol. 33.
Parkhill et al.; “The Genome Sequence of the Food-Borne PathogenCampylobacter jejuniReveals Hypervariable Sequences”;Nature: Feb. 10, 2000; pp. 665-668: vol. 403.
Penner et al.; “Diversity of Lipopolysaccharide Structures InCampylobacter jejuni”;The Journal of Infectious Diseases; Dec. 1997; pp. S135-S138; vol. 176, No. 2.
Prendergast et al.; “Lipoplysaccharides fromCampylobacter jejuniO:41 Strains Associated with Guillain-BarnéSyndrome Exhibit Mimicry of GM1 Ganglioside”;Infection and Immunity: Aug. 1998; pp. 3649-3677; vol. 66, No. 6.
Preston et al.; “The Lipooligosaccharides of Pathogenic Gram-Negative Bacteria”;Critical Reviews in Microbiology: 1996: pp. 139-180: vol. 22. No. 3.
Sabesan et al.: “Combined Chemical and Enzymatic Synthesis of Sialyloligosaccharides and Characterization by 500-MHz1H and C NMR Spectroscopy”;J. Am. Chem. Soc.: 1986; pp. 2066-2080; vol. 108.
Salloway et al.; “Miller-Fisher Syndrome Associated withCampylobacter jejuniBearing Lipopolysaccharide Molecules that Mimic Human Ganglioside GD”;Infect. Immun.: 1996; pp. 2945-2949; vol. 64.
Wakarchuk et al.; “Functional Relationships of the Genetic Locus Encoding the Glycosyltransferase Enzymes Involved In Expression of the Lacto-N-neotetraose Terminal Lipopolysaccharide Structure inNeisseria meningitidis”;J. Biol. Chem.: 1996: pp. 19168-19173; vol. 271.
Wood et al.; “Cloning, Mutation and Distribution of a Putative Lipoplysaccharide Biosynthesis Locus inCampylobacter jejuni”;Microbiology: Feb. 1999; pp. 379-388; vol. 145, No. 2.

Affiliated with

Also associated with

Rating

Nucleic acids encoding sialytranferases from C. jejuni does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acids encoding sialytranferases from C. jejuni, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acids encoding sialytranferases from C. jejuni will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3794737