Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2007-03-13
2007-03-13
Ibrahim, Medina A. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C800S298000, C536S023600
Reexamination Certificate
active
10871841
ABSTRACT:
The present invention provides a polynucleotide sequence encoding a component of the protein machinery involved in small RNA trafficking,Cucurbita maximaphloem small RNA-binding protein (CmPSRB 1), and the corresponding polypeptide sequence. The invention also provides genetic constructs and transgenic plants comprising the polynucleotide sequence encoding a phloem small RNA-binding protein to alter (e.g., prevent, reduce or elevate) non-cell autonomous signaling events in the plants involving small RNA metabolism. These signaling events are involved in a broad spectrum of plant physiological and biochemical processes, including, for example, systemic resistance to pathogens, responses to environmental stresses, e.g., heat, drought, salinity, and systemic gene silencing (e.g., viral infections).
REFERENCES:
Aoki, Koh et al., (2002) “A Subclass of Plant Heat Shock Cognate 70 Chaperones Carries A Motif That Facilitates Trafficking Through Plasmodesmata”PNAS, 99(25):16342-16347.
Atkins, C. A. (1999) Spontaneous Phloem Exudation Accompanying Abscission inLupinus mutabilis(Sweet)J. Exp. Bot.50, 805-812.
Balachandran, S. et al., (1997) Phloem Sap Proteins fromCucurbita maximaandRicinus communisHave the Capacity to Traffic Cell to Cell through PlasmodesmataProc. Natl. Acad. Sci. U.S.A.94, 14150-14155.
Bernstein, E. et al., (2001) “Role for a Bidentate Ribonuclease in the Initiation Step of RNA Interference”Nature409, 363-366.
Carrington, J. C. et al., (1996) “Cell to Cell and Long Distance Transport of Viruses in Plants”Plant Cell8, 1669-1681.
Dalmay, T. et al., (2000) “An RNA-Dependent RNA Polymerase Gene in Arabidopsis is Required for Posttranscriptional Gene Silencing Mediated by a Transgene but not by a Virus”Cell101, 543-553.
Dalmay, T. et al., (2001) “SDE3 Encodes an RNA Helicase Required for Posttranscriptional Gene Silencing in Arabidopsis”EMBOJ.20(8): 2069-2077.
Di Serio, F. et al., (2001) “Sense- and Antisense-Mediated Gene Silencing in Tobacco is Inhibited by the Same Viral Suppressors and is associated With Accumulation of Small RNAs”Proc. Natl. Acad. Sci.98(11): 6506-6510.
Elbashir, M. et al., (2002) “Analysis of Gene Function in Somatic Mammalian Cells Using Small Interfering RNAs”Methods26, 199-213.
Elbashir, S. M. (2001) “RNA Interference is Mediated by 21 and 22-Nucleotide RNAs”Genes Dev.15, 188-200.
Fagard, M. et al., (2000) (Trans) Gene Silencing in Plants: How Many Mechanisms?Annu Rev. Plant Physiol. Plant Mol. Biol.51, 167-194.
Fire, et al., (1998) “Potent and Specific Genetic Interference by Double-Stranded RNA inCaenorhabditis elegans” Nature391, 806-811.
Gilbertson, R. L. et al., (1996) How Do Viruses Traffic on the ‘Vascular Highway’?Trends Plant Sci.1(8):260-268.
Gou, H. S. et al., (2002) “A Viral Protein Inhibits the Long Range Signaling Activity of the Gene Silencing Signal”EMBOJ.21(3):398-407.
Hamilton, A. J. et al., (2002) Two Classes of Short Interfering RNA in RNA SilencingEMBOJ.21(17): 4671-4679.
Hamilton, A. J. et al., (1999) “A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants”Science286, 950-952.
Hammond, S. M. et al., (2000) “An RNA Directed Nuclease Meditates Post-Transcriptional Gene Silencing inDrosophilaCells”Nature404, 293-296.
Hannon, G. J. (2002) “RNA Interference”Nature418, 244-251.
Hartono, S. J. (2003) “Nucleotide Sequence and Genome Organization of Cucumber Yellows Virus, a Member of the Genus Crinivirus”Gen. Virol.84, 1007-1012.
Havey, M. J. et al., (1998) Differential Transmission of theCucumisOrganellar GenomesTheor. Applied Genet.97, 122-128.
Haywood, V. et al., (2002) “Plasmosdesmata: Pathways for Protein and Ribonucleoprotein Signaling”Plant Cell14, S303-S325.
Hutvagner, G. et al., (2001) “A Cellular Function for the RNA Interference Enzyme Dicer in the Maturation of thelet-7Small Temporal RNA”Science293, 834-838.
Hutvagner, G. et al., (2002) “A microRNA in a Multiple Turnover RNAi Enzyme Complex”Science297, 2056-2060.
Jackson, D. (2001) “The Long and the Short of it: Signaling Development through Plasmodesmata”Plant Cell13, 2569-2571.
Jeschke, W. D. et al., (1991) “Cation and Chloride Partitioing through Xylem and Phloem within the Whole PlantRicinus communis L.Under Conditions of Salt Stress”J. Exp. Bot.42(242): 1105-1116.
Jorgensen, R. A. et al., (1998) “An RNA-Based Information Superhighway in Plants”Science279 (5356): 1486-1487.
Kim, J. Y. et al., (2001) “Development Changes Due to Long-Distance Movement of a Homeobox Fusion Transcript in Tomato”Science293, 287-289.
Kim, M. et al., (2002) “Intercellular Trafficking of a KNOTTED 1 Green Fluorescent Fusion in the Leaf and Shoot meristem ofArabidopsis” Proc. Natl. Acad. Sci. USA99(6): 4103-4108.
Kovalchuk, I. et al., (2003) “Pathogen-Induced Systemic Plant Signal Triggers DNA Rearrangements”Nature423, 760-762.
Llave, C. et al., (2002) “Endogenous and Silencing Associated Small RNAs in Plants”Plant Cell14, 1605-1619.
Llave, C. et al., (2002) “Cleavage of Scarecrow-Like mRNA Targets Directed by a Class ofArabidopsismiRNA”Science297, 2053-2056.
Lucas, W. J. et al., (1995) “Selective Trafficking of KNOTTED1 Homeodomain Protein and its mRNA Through Plasmodesmata”Science270, 1980-1983.
Lucas, W. J. et al., (2001) “RNA as a Long-Distance Information Macromolecule in Plants”Nat. Rev. Mol. Cell Biol.2, 849-857.
Mallory, A. C. et al., (2001) “HC-Pro Suppression of Transgene Silencing Eliminates the Small RNAs but not Transgene Methylation or the Mobile Signal”Plant Cell13, 571-583.
Matzke, M. et al., (2001) “RNA: Guiding Gene Silencing”Science293, 1080-1083.
Mlotshwa, S. et al., (2002) “RNA Silencing and the Mobile Silencing Signal”Plant Cell14, S289-S301.
Mourrain, P. et al., (2000) “ArabidopsisSGS2 and SGS3 Genes are Required for Posttranscriptional Gene Silencing and Natural Virus Resistance”Cell101, 533-542.
Nakajima, K. et al., (2001) “Intercellular Movement of the Putative Transcription Factor SHR in Root Patterning”Nature413, 307-311.
Olsen, P. H. et al., (1999) “The lin-4 Regulatory RNA Controls Developmental Timing inCaenorhabditis elegansby Blocking LIN-14 Protein Synthesis after the Initiation of Translation”Dev. Biol.216, 671-680.
Palauqui, J.-C et al., (1997) “Systematic Acquired Silencing: Transgene-Specific Post-Transcriptional Silencing is Transmitted by Grafting from Silenced Stocks to non-Silenced Scions”EMBOJ16(15): 4738-4745.
Pang, S. Z. et al., (2000) “Resistance to Squash Mosaic Comovirus in Transgenic Squash Plants expressing its Coat Protein Genes”Mol. Breeding6, 87-93.
Reinhart, B. J. et al., (2002) “MicroRNAs in Plants”Genes Dev.16, 1616-1626.
Robards, A. W. et al., (1990) “Plasmodesmata”Annu Rev. Plant Phys. Plant Mol. Biol.41, 369-419.
Roignant, et al., (2003) “Absence of Transitive and Systematic Pathways Allows Cell-Specific and Isoform-Specific RNAi inDrosophila” RNA9, 299-309.
Rojas, M. R. et al., (1997) “Capsid Protein and Helper Component-Proteinase Function as Potyvirus Cell-to-Cell Movement Proteins”Virology237, 283-295.
Ruiz-Medrano, R. (1999) “Phloem Long Distance Transport of CmNACP mRNA: Implications for Supracellular Regulations in Plants”Development126, 4405-4419.
Schiefelbein, J. (2003) “Cell-Fate Specification in the Epidermis: A Common Patterning Mechanism in the Root and Shoot”Curr. Opin. Plant Biol.6, 74-78.
Sessions, A. et al., (2000) “Cell—Cell Signaling and Movement by the Floral Transcription Factors LEAFY and APETALA 1”Science289, 779-781.
Sijen, T. et al., (2001) “On the Role of RNA Amplification in dsR
Lough Tony J.
Lucas William J.
Varkonyi-Gasic Erika
Yoo Byung-Chun
Agrigenesis Biosciences Limited
Ibrahim Medina A.
Morrison & Foerster / LLP
The Regents of the University of California
LandOfFree
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