Nucleic acids encoding oxidation-reduction sensitive green...

Details Nucleic acids encoding oxidation-reduction sensitive green... Nucleic acids encoding oxidation-reduction sensitive green...

2008-01-01

2008-01-01

Robinson, Hope (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S006120, C435S007100, C435S320100, C435S252000, C435S325000, C536S023100, C530S350000

Reexamination Certificate

active

11255677

ABSTRACT:
The disclosure provides proteins that can be used to determine the redox status of an environment (such as the environment within a cell or subcellular compartment). These proteins are green fluorescent protein (GFP) variants (also referred to as redox sensitive GFP (rosGFP) mutants), which have been engineered to have two cysteine amino acids near the chromophore and within disulfide bonding distance of each other. Also provided are nucleic acid molecules that encode rosGFPs, vectors containing such encoding molecules, and cells transformed therewith. The disclosure further provides methods of using the rosGFPs (and encoding molecules) to analyze the redox status of an environment, such as a cell, or a subcellular compartment within a cell. In certain embodiments, both redox status and pH are analyzed concurrently.

REFERENCES:
patent: 5565323 (1996-10-01), Parker et al.
patent: 5625048 (1997-04-01), Tsien et al.
patent: 5777079 (1998-07-01), Tsien et al.
patent: 5804387 (1998-09-01), Cormack et al.
patent: 5874304 (1999-02-01), Zolotukhin et al.
patent: 5888498 (1999-03-01), Davis et al.
patent: 5908747 (1999-06-01), Shibata et al.
patent: 5912137 (1999-06-01), Tsien et al.
patent: 5968738 (1999-10-01), Anderson et al.
patent: 5968750 (1999-10-01), Zolotukhin et al.
patent: 5994077 (1999-11-01), Valdivia et al.
patent: 6020192 (2000-02-01), Muzyczka et al.
patent: 6054321 (2000-04-01), Tsien et al.
patent: 6066476 (2000-05-01), Tsien et al.
patent: 6077707 (2000-06-01), Tsien et al.
patent: 6090919 (2000-07-01), Cormack et al.
patent: 6096865 (2000-08-01), Michaels
patent: 6124128 (2000-09-01), Tsien et al.
patent: 6140132 (2000-10-01), Tsien et al.
patent: 6146826 (2000-11-01), Chalfie et al.
patent: 6172188 (2001-01-01), Thastrup et al.
patent: 6306600 (2001-10-01), Kain et al.
patent: 6316181 (2001-11-01), Fillmore et al.
patent: 6319669 (2001-11-01), Tsien et al.
patent: 6342379 (2002-01-01), Tsien et al.
patent: 6414119 (2002-07-01), Fisher
patent: 6509174 (2003-01-01), Jordan et al.
patent: 6803188 (2004-10-01), Tsien et al.
patent: 2002/0123113 (2002-09-01), Tsien et al.
patent: 2003/0017538 (2003-01-01), Miyawaki et al.
patent: WO 96/23810 (1996-08-01), None
patent: WO 98/02571 (1998-01-01), None
patent: WO 99/64592 (1999-12-01), None
Akerley et al., “Systematic identification of essential genes by in vitromarinermutagenesis,”Proc. Natl. Acad. Sci. USA, 95:8927-8932, Jul. 1998.
Arai et al., “Mitochondrial Phospholipid Hydroperoxide Glutathione Peroxidase Plays a Major Role in Preventing Oxidative Injury to Cells,”J. Biological Chemistry, 274(8):4924-4933, 1999.
Chattoraj et al., “Ultra-fast excited dynamics in green fluorescent protein: Multiple states and proton transfer,”Proc. Natl. Acad. Sci. USA, 93:8362-8367, Aug. 1996.
Coxon and Bestor, “Proteins that glow in green and blue,”Chemistry&Biology, 2:119-121, 1995.
Gustafsson et al., “Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes,”Nucleic Acids Research, 24(19):3756-3762, 1996.
Inouye and Tsuji, “Evidence for redox forms of theAequoreagreen fluorescent protein,”FEBS Letters351:211-214, 1994.
Náray-Fejes-Tóth et al., “Subcellular Localization of the Type 2 11β-Hydroxysteriod Dehydrogenase,”J. Biological Chemistry, 271(26):15436-15442, 1996.
Niwa et al., “Chemical nature of the light emitter of theAequoreagreen fluorescent protein,”Proc. Natl. Acad. Sci.USA, 93:13617-13622, Nov. 1996.
Li et al., “Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter,”J. Biological Chemistry, 273(52):34970-34975, 1998.
“Living Colors® User Manual,”Clontech, 51 pp., Apr. 2, 1999.
Okamato et al., “Redox-dependent Regulation of Nuclear Import of the Glucocorticoid Receptor,”J. Biological Chemistry, 274(15):10363-10371, 1999.
Reichel et al., Enhanced green fluorescence by the expression of anAequorea victoriagreen fluorescent protein mutant in mono- and dicotyledonous plant cells,Pro. Natl. Acad. Sci.USA, 93:5888-5893, Jun. 1996.
Siemering et al., “Mutations that suppress the thermosensitivity of green fluorescent protein,”Current Biology, 6(12):1653-1663, 1996.
Simpson et al., “Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing,”EMBO Reports, 1(3):287-292, 2000.
Stearns, “The green revolution,”Current Biology, 5:262-264, 1995.
Wachter and Remington, “Sensitivity of the yellow variant of green fluorescent protein to halides and nitrate,”Current Biology9(17):628-629, 1999.
“Aequorea victoriagreen-fluorescent protein mRNA complete cds” GenBank Accession M62653, Apr. 26, 1993.
“Aequorea victoriagreen-fluorescent protein mRNA complete cds,” GenBank Accession M62654, Apr. 26, 2003.
“Clontech GFP License Statements,”Clontech, .clontech.com/gfp/license/index.html, 2 pp., accessed Nov. 26, 2000.
“The Fluorophore of Green Fluorescent Protein,” pps99.cryst.bbk.ac.uk/projects/gmocz/gfp.htm, 6 pp., accessed Nov. 18, 2000.
“Illuminating the Structure of Green Fluorescent Protein,”NPACI Online, www.npaci.edu/online/v4.14/gfp.html, IV:14, 4 pp., accessed Jul. 12, 2000.
“Living Colors™ Fluorescent Proteins,”Clontech, www.clontech.com/gfp/, accessed Nov. 18, 2000.
“Living Colors™ Fluorescent Timer,”Clontech, presort mail advertisement.
“Patent mutations for improved performance,”Amersham Biosciences, www5.amershambiosciences.com/aptrix/upp00919.nsf/content/D9B3F40CB359B9B, 5 pp., accessed Mar. 12, 2003.
“Product Catalog: N-Terminal Enhanced Fluorescent Protein Vectors,”Clontech, www.clontech.com/products/catalog01/Sec5/p178nterminalefpv.html, 3 pp., accessed Jan. 13, 2001.

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