Nucleic acids encoding mutant urease

Details Nucleic acids encoding mutant urease Nucleic acids encoding mutant urease

1997-11-11

1998-07-21

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 43525233, 536 232, C12N 121, C12N 1563, C07H 2104

Patent

active

057834369

ABSTRACT:
A mutant urease of Klebsiella aerogenes characterized as .alpha.H219 having a glutamine (Q) in position 219 in place of histidine (H). The resulting enzyme has a low affinity for substrate (high value of K.sub.m) and is particularly useful in assays and test kits for measuring urea concentrations in body fluids.

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